Porous thermoresponsive-co-biodegradable hydrogels as tissue-engineering scaffolds for 3-dimensional in vitro culture of chondrocytes

Xiao Huang, Yue Zhang, Henry J. Donahue, Tao Lowe

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

A new porous, thermoresponsive, partially biodegradable, chemically crosslinked hydrogel system was developed, characterized, and tested as a cartilage tissue-engineering scaffold for in vitro chondrocyte culture over a 4-week period. The hydrogel system was composed of poly(N-isopropylacrylamide), poly(D,L-lactic acid), and dextran segments. Pores in the hydrogels were generated using a salt leaching technique. The hydrogels showed thermoresponsive properties, with a lower critical solution temperature at approximately 32°C. They continuously swelled at physiological temperature in phosphate buffered saline (pH 7.4) for at least 1 month. Chondrocytes isolated from embryonic chick sterna were seeded into the hydrogel scaffolds at room temperature and cultured at 37°C for 4 weeks. Real-time reverse- transcriptase polymerase chain reaction quantification was conducted every week to study messenger ribonucleic acid levels of 3 chondrocyte phenotypic markers: type II collagen, type X collagen, and Indian hedgehog. Results suggested that chondrocytes maintained their phenotype during the 4-week in vitro culture and could mimic in vivo development. Chondrocytes were non-enzymatically harvested from the hydrogel scaffold at the end of the fourth week by simply lowering the temperature from 37°C to room temperature. The harvested chondrocytes kept a round morphology, confirming the maintenance of the chondrocyte phenotype in the hydrogel scaffolds.

Original languageEnglish (US)
Pages (from-to)2645-2652
Number of pages8
JournalTissue Engineering
Volume13
Issue number11
DOIs
StatePublished - Nov 1 2007
Externally publishedYes

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Scaffolds (biology)
Tissue engineering
Hydrogels
Scaffolds
Collagen
Temperature
Dextran
Polymerase chain reaction
Cartilage
Lactic acid
RNA
Leaching
Phosphates
Salts

All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Porous thermoresponsive-co-biodegradable hydrogels as tissue-engineering scaffolds for 3-dimensional in vitro culture of chondrocytes. / Huang, Xiao; Zhang, Yue; Donahue, Henry J.; Lowe, Tao.

In: Tissue Engineering, Vol. 13, No. 11, 01.11.2007, p. 2645-2652.

Research output: Contribution to journalArticle

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