Post-translational glycosylation of target proteins implicate molecular mimicry in the pathogenesis of HTLV-1 associated neurological disease

Sang Lee, Yoojin Shin, Jacob Marler, Michael C. Levin

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We hypothesized that molecular mimics between human T lymphotropic virus type 1 (HTLV-1) and human autoantigens were based upon post-translational modification of target proteins. Proteins purified from MT-2 (HTLV-1 infected) and Jurkat (HTLV-1 negative) cells were used for western blotting with HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP) IgG. In contrast to normal IgG, HAM/TSP IgG immunoreacted with proteins in MT-2 cells at 22-24 kDa, pI 8.0, which were identified as peroxiredoxin 1 and HTLV-1-p24-(gag) by mass spectroscopy. Western blots following glycoprotein purification showed that HAM/TSP IgG reacted with PrX-1 and p24 in MT-2 cells but not in Jurkat cells, indicating that the mimicking target proteins were glycosylated. These data suggest that post-translational glycosylation of target proteins may play a role in the pathogenesis of HAM/TSP.

Original languageEnglish (US)
Pages (from-to)140-148
Number of pages9
JournalJournal of Neuroimmunology
Volume204
Issue number1-2
DOIs
StatePublished - Nov 15 2008

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Molecular Mimicry
Human T-lymphotropic virus 1
Tropical Spastic Paraparesis
Glycosylation
Spinal Cord Diseases
Immunoglobulin G
Western Blotting
Peroxiredoxins
Proteins
Jurkat Cells
Autoantigens
Post Translational Protein Processing
Mass Spectrometry
Glycoproteins

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

Cite this

Post-translational glycosylation of target proteins implicate molecular mimicry in the pathogenesis of HTLV-1 associated neurological disease. / Lee, Sang; Shin, Yoojin; Marler, Jacob; Levin, Michael C.

In: Journal of Neuroimmunology, Vol. 204, No. 1-2, 15.11.2008, p. 140-148.

Research output: Contribution to journalArticle

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