Postischemic hyperthermia increases expression of hsp72 mRNA after brief ischemia in the gerbil

Sadao Suga, Thaddeus Nowak

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Brain temperature during ischemia critically determines insult severity, and temperature changes during recirculation may also affect subsequent injury. We have examined the impact of postischemic temperature on induction of the 70 kDa stress protein, hsp72, after brief ischemia in the gerbil. Animals were subjected to 2 min ischemia after which they were maintained under continuous halothane anesthesia during 3 h recirculation, and were either kept normothermic or subjected to hyperthermia comparable to that which occurs spontaneously in gerbils released from anesthesia immediately after the occlusion. Quantitative in situ hybridization showed striking dependence of hsp72 induction on postischemic hyperthermia. This result establishes that delayed temperature-sensitive signals mediate this injury- associated transcriptional response, and demonstrates that postischemic temperature must be carefully monitored in studies of gene expression and induced tolerance employing brief ischemic insults.

Original languageEnglish (US)
Pages (from-to)57-60
Number of pages4
JournalNeuroscience Letters
Volume243
Issue number1-3
DOIs
StatePublished - Feb 27 1998
Externally publishedYes

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Gerbillinae
Fever
Ischemia
Messenger RNA
Temperature
Anesthesia
Wounds and Injuries
Halothane
Heat-Shock Proteins
In Situ Hybridization
Gene Expression
Brain

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Postischemic hyperthermia increases expression of hsp72 mRNA after brief ischemia in the gerbil. / Suga, Sadao; Nowak, Thaddeus.

In: Neuroscience Letters, Vol. 243, No. 1-3, 27.02.1998, p. 57-60.

Research output: Contribution to journalArticle

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