Posttranscriptional regulation of the expression of CAD gene during differentiation of F9 teratocarcinoma cells by induction with retinoic acid and dibutyryl cyclic AMP

Rao Gadiparthi, Robert L. Church, Jeffrey N. Davidson

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We have studied the regulation of expression of the carbamoyl-phosphate synthetase II-aspartate transcarbamylase-dihydroorotase gene in F9 teratocarcinoma cells during their differentiation into parietal endoderm cells by induction with a combination of retinoic acid and dibutyryl cyclic AMP. Steady-state levels of CAD mRNA decreased by 7-fold in F9 cells following 120 h of retinoic acid and dibutyryl cyclic AMP induction as compared to levels in uninduced cells. Conversely, no apparent changes were found in the steady-state levels of β-actin mRNA between induced and uninduced cells. Despite a 7-fold decrease in the steady-state levels of CAD mRNA, its rate of transcription remained the same between induced and uninduced cells, indicating a role for posttranscriptional mechanisms for its down regulation during retinoic acid- and dibutyryl cyclic AMP-induced differentiation of F9 cells. The cellular growth rate of F9 cells as determined by [3H]thymidine uptake and parallel cell counting decreased markedly during their induction with retinoic acid and dibutyryl cyclic AMP. Taken together, it is apparent that the expression of the CAD gene is cell-growth-dependent and its regulation in this system is at the posttranscriptional level.

Original languageEnglish (US)
Pages (from-to)238-242
Number of pages5
JournalFEBS Letters
Volume232
Issue number1
DOIs
StatePublished - May 9 1988

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Teratocarcinoma
Bucladesine
Gene Expression Regulation
Tretinoin
Computer aided design
Genes
Messenger RNA
Dihydroorotase
Aspartate Carbamoyltransferase
Carbamyl Phosphate
Cell growth
Transcription
Ligases
Thymidine
Actins
Endoderm
Growth
Cell Differentiation
Down-Regulation
Gene Expression

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Posttranscriptional regulation of the expression of CAD gene during differentiation of F9 teratocarcinoma cells by induction with retinoic acid and dibutyryl cyclic AMP. / Gadiparthi, Rao; Church, Robert L.; Davidson, Jeffrey N.

In: FEBS Letters, Vol. 232, No. 1, 09.05.1988, p. 238-242.

Research output: Contribution to journalArticle

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