Posttranslational processing of SREBP-1 in rat hepatocytes is regulated by insulin and cAMP

Chandrahasa R. Yellaturu, Xiong Deng, Lauren M. Cagen, Henry G. Wilcox, Edwards Park, Rajendra Raghow, Marshall Elam

Research output: Contribution to journalArticle

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Abstract

Insulin and cAMP have opposing effects on de novo fatty acid synthesis in liver and in cultured hepatocytes mediated by sterol-regulatory element binding protein (SREBP). To determine whether these agents regulate the cleavage of full-length SREBP to generate the transcriptionally active N-terminal fragment (nSREBP) in primary rat hepatocytes, an adenoviral vector (Ad-SREBP-1a) was constructed to constitutively express full-length SREBP-1a. Insulin increased, and dibutyryl (db)-cAMP inhibited, generation of nSREBP-1a from its full-length precursor. Insulin stimulated processing of SREBP-1a within 1 h, and the effect was sustained for at least 24 h. The initial stimulation of SREBP processing by insulin preceded measurable reduction in Insig-2 mRNA levels. Rat hepatocytes were also infected with an adenovirus expressing the nuclear form of SREBP-1c (Ad-nSREBP-1c). Insulin increased the half-life of constitutively expressed nSREBP-1c, and this effect of insulin was also inhibited by db-cAMP.

Original languageEnglish (US)
Pages (from-to)174-180
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume332
Issue number1
DOIs
StatePublished - Jun 24 2005

Fingerprint

Sterol Regulatory Element Binding Protein 1
Rats
Hepatocytes
Sterol Regulatory Element Binding Proteins
Insulin
Processing
Adenoviridae
Liver
Half-Life
Fatty Acids
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Posttranslational processing of SREBP-1 in rat hepatocytes is regulated by insulin and cAMP. / Yellaturu, Chandrahasa R.; Deng, Xiong; Cagen, Lauren M.; Wilcox, Henry G.; Park, Edwards; Raghow, Rajendra; Elam, Marshall.

In: Biochemical and Biophysical Research Communications, Vol. 332, No. 1, 24.06.2005, p. 174-180.

Research output: Contribution to journalArticle

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