Pouch tissue and angiotensin peptide generation

Laxmansa C. Katwa, Yao Sun, Scott E. Campbell, Suresh C. Tyagi, Arvinder K. Dhalla, Jagan C. Kandala, Karl Weber

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Myofibroblasts and their potential to generate angiotensin (Ang) II and transforming growth factor beta 1 (TGF-β1) at sites of infarction in the rat heart have been implicated in tissue repair. These cells likewise contribute to repair in a subcutaneous pouch model of fibrous tissue formation. Their appearance in pouch tissue coincides with high density ACE and Ang II receptor binding, suggesting a role for Ang II in tissue repair. Using pouch tissue studied at different time points of repair, the present study examined the expression of requisite mRNA for Ang peptide generation: angiotensinogen, Ao; an aspartyl protease, either cathepsin-D, Cat-D, or renin; and angiotensin converting enzyme, ACE. TGF-β1 and type I collagen mRNA expression was also addressed. Unlike pouch studied on day 2 and 4, at 7, 14 and 21 days, we found: (a) expression of Ao, Cat-D but not renin, ACE and TGF-β1 mRNA; (b) Ang I and Ang II peptides in pouch tissue and exudate; (c) the presence of Cat-D activity but no renin activity; (d) an increase in type I collagen mRNA with time; (e) upregulation of pouch tissue ACE mRNA expression by lisinopril treatment, whereas AT1 and AT2 receptor antagonists (losartan and PD 123177, respectively) downregulated the expression of mRNA for ACE, when compared to untreated controls; (f) downregulation of TGF-β1 mRNA expression by lisinopril and losartan compared to untreated controls; and (g) PD 123177 had no effect, whereas lisinopril and losartan treatment significantly (P < 0.05) reduced type I collagen mRNA expression. Thus, in this model of fibrous tissue formation, we found expression of component genes involved in Ang peptide (I and II) and TGF-β1 generation and Ang II upregulation of TGF-β1 expression, suggesting Ang II and/or TGF-β1 may upregulate type I collagen expression during tissue repair. Pharmacologic intervention studies with lisinopril or losartan indicate Ang II plays a role in the reciprocal regulation of ACE mRNA expression, which modulates Ang II levels at sites of repair.

Original languageEnglish (US)
Pages (from-to)1401-1413
Number of pages13
JournalJournal of Molecular and Cellular Cardiology
Volume30
Issue number7
DOIs
StatePublished - Jan 1 1998
Externally publishedYes

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Angiotensins
Angiotensin II
Transforming Growth Factor beta
Messenger RNA
Peptides
Lisinopril
Losartan
Collagen Type I
Renin
Angiotensin I
Cats
Up-Regulation
Down-Regulation
Aspartic Acid Proteases
Gene Components
Transforming Growth Factor beta1
Angiotensinogen
Cathepsin D
Angiotensin Receptors
Myofibroblasts

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

Katwa, L. C., Sun, Y., Campbell, S. E., Tyagi, S. C., Dhalla, A. K., Kandala, J. C., & Weber, K. (1998). Pouch tissue and angiotensin peptide generation. Journal of Molecular and Cellular Cardiology, 30(7), 1401-1413. https://doi.org/10.1006/jmcc.1998.0708

Pouch tissue and angiotensin peptide generation. / Katwa, Laxmansa C.; Sun, Yao; Campbell, Scott E.; Tyagi, Suresh C.; Dhalla, Arvinder K.; Kandala, Jagan C.; Weber, Karl.

In: Journal of Molecular and Cellular Cardiology, Vol. 30, No. 7, 01.01.1998, p. 1401-1413.

Research output: Contribution to journalArticle

Katwa, LC, Sun, Y, Campbell, SE, Tyagi, SC, Dhalla, AK, Kandala, JC & Weber, K 1998, 'Pouch tissue and angiotensin peptide generation', Journal of Molecular and Cellular Cardiology, vol. 30, no. 7, pp. 1401-1413. https://doi.org/10.1006/jmcc.1998.0708
Katwa LC, Sun Y, Campbell SE, Tyagi SC, Dhalla AK, Kandala JC et al. Pouch tissue and angiotensin peptide generation. Journal of Molecular and Cellular Cardiology. 1998 Jan 1;30(7):1401-1413. https://doi.org/10.1006/jmcc.1998.0708
Katwa, Laxmansa C. ; Sun, Yao ; Campbell, Scott E. ; Tyagi, Suresh C. ; Dhalla, Arvinder K. ; Kandala, Jagan C. ; Weber, Karl. / Pouch tissue and angiotensin peptide generation. In: Journal of Molecular and Cellular Cardiology. 1998 ; Vol. 30, No. 7. pp. 1401-1413.
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abstract = "Myofibroblasts and their potential to generate angiotensin (Ang) II and transforming growth factor beta 1 (TGF-β1) at sites of infarction in the rat heart have been implicated in tissue repair. These cells likewise contribute to repair in a subcutaneous pouch model of fibrous tissue formation. Their appearance in pouch tissue coincides with high density ACE and Ang II receptor binding, suggesting a role for Ang II in tissue repair. Using pouch tissue studied at different time points of repair, the present study examined the expression of requisite mRNA for Ang peptide generation: angiotensinogen, Ao; an aspartyl protease, either cathepsin-D, Cat-D, or renin; and angiotensin converting enzyme, ACE. TGF-β1 and type I collagen mRNA expression was also addressed. Unlike pouch studied on day 2 and 4, at 7, 14 and 21 days, we found: (a) expression of Ao, Cat-D but not renin, ACE and TGF-β1 mRNA; (b) Ang I and Ang II peptides in pouch tissue and exudate; (c) the presence of Cat-D activity but no renin activity; (d) an increase in type I collagen mRNA with time; (e) upregulation of pouch tissue ACE mRNA expression by lisinopril treatment, whereas AT1 and AT2 receptor antagonists (losartan and PD 123177, respectively) downregulated the expression of mRNA for ACE, when compared to untreated controls; (f) downregulation of TGF-β1 mRNA expression by lisinopril and losartan compared to untreated controls; and (g) PD 123177 had no effect, whereas lisinopril and losartan treatment significantly (P < 0.05) reduced type I collagen mRNA expression. Thus, in this model of fibrous tissue formation, we found expression of component genes involved in Ang peptide (I and II) and TGF-β1 generation and Ang II upregulation of TGF-β1 expression, suggesting Ang II and/or TGF-β1 may upregulate type I collagen expression during tissue repair. Pharmacologic intervention studies with lisinopril or losartan indicate Ang II plays a role in the reciprocal regulation of ACE mRNA expression, which modulates Ang II levels at sites of repair.",
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