Preliminary analysis of the mouse cerebellum proteome

Sarka Beranova, Michael J. Pabst, Tara M. Russell, Francesco Giorgianni, Daniel Goldowitz, Dominic M. Desiderio

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

This paper reports on the initial analysis of protein expression in the mouse cerebellum with the proteomics approach. Proteins from cerebellar tissue homogenates were separated by two-dimensional gel electrophoresis, and the proteins were stained with colloidal Coomassie Blue to produce a high-resolution map of the cerebellum proteome. Selected proteins from this map were digested with trypsin, and the resulting tryptic peptides were analyzed by matrix-assisted laser desorption/ionization mass spectrometry and liquid chromatography-electrospray quadrupole ion trap mass spectrometry. The mass spectrometric data were used to identify the proteins through searches of the SWISSPROT protein sequence database. To date, 30 prominent proteins with various functional characteristics were identified. These data will be used for future studies of differential protein expression in mouse models of neurological disorders.

Original languageEnglish (US)
Pages (from-to)135-140
Number of pages6
JournalMolecular Brain Research
Volume98
Issue number1-2
DOIs
StatePublished - Jan 31 2002

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Proteome
Cerebellum
Proteins
Protein Databases
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Electrophoresis, Gel, Two-Dimensional
Nervous System Diseases
Liquid Chromatography
Proteomics
Trypsin
Mass Spectrometry
Ions
Peptides

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Preliminary analysis of the mouse cerebellum proteome. / Beranova, Sarka; Pabst, Michael J.; Russell, Tara M.; Giorgianni, Francesco; Goldowitz, Daniel; Desiderio, Dominic M.

In: Molecular Brain Research, Vol. 98, No. 1-2, 31.01.2002, p. 135-140.

Research output: Contribution to journalArticle

Beranova, Sarka ; Pabst, Michael J. ; Russell, Tara M. ; Giorgianni, Francesco ; Goldowitz, Daniel ; Desiderio, Dominic M. / Preliminary analysis of the mouse cerebellum proteome. In: Molecular Brain Research. 2002 ; Vol. 98, No. 1-2. pp. 135-140.
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