Premature termination of GAT1 transcription explains paradoxical negative correlation between nitrogen-responsive mRNA, but constitutive low-level protein production

Georis Isabelle, Jennifer J. Tate, Fabienne Vierendeels, Terrance Cooper, Evelyne Dubois

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The first step in executing the genetic program of a cell is production of mRNA. In yeast, almost every gene is transcribed as multiple distinct isoforms, differing at their 5' and/or 3' termini. However, the implications and functional significance of the transcriptome-wide diversity of mRNA termini remains largely unexplored. In this paper, we show that the GAT1 gene, encoding a transcriptional activator of nitrogen-responsive catabolic genes, produces a variety of mRNAs differing in their 5' and 3' termini. Alternative transcription initiation leads to the constitutive, low level production of 2 full length proteins differing in their N-termini, whereas premature transcriptional termination generates a short, highly nitrogen catabolite repression- (NCR-) sensitive transcript that, as far as we can determine, is not translated under the growth conditions we used, but rather likely protects the cell from excess Gat1.

Original languageEnglish (US)
Pages (from-to)824-837
Number of pages14
JournalRNA Biology
Volume12
Issue number8
DOIs
StatePublished - Jan 1 2015

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Nitrogen
Messenger RNA
Genes
Catabolite Repression
Proteins
Transcriptome
Protein Isoforms
Yeasts
Growth

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Premature termination of GAT1 transcription explains paradoxical negative correlation between nitrogen-responsive mRNA, but constitutive low-level protein production. / Isabelle, Georis; Tate, Jennifer J.; Vierendeels, Fabienne; Cooper, Terrance; Dubois, Evelyne.

In: RNA Biology, Vol. 12, No. 8, 01.01.2015, p. 824-837.

Research output: Contribution to journalArticle

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