Prenatal diagnosis with fetal cells isolated from maternal blood by multiparameter flow cytometry

James O. Price, Sherman Elias, Stephen S. Wachtel, Katherine Klinger, Michael Dockter, Avirachan Tharapel, Lee P. Shulman, Owen Phillips, Carole M. Meyers, Donna Shook, Joe Leigh Simpson

Research output: Contribution to journalArticle

220 Citations (Scopus)

Abstract

A long-sought goal of medical genetics has been development of prenatal diagnostic procedures that do not endanger the conceptus. Reliable and universal screening for cytogenetic disorders would require analysis of fetal cells isolated from the maternal circulation. This would be applicable to all pregnant women, irrespective of their ages or histories. In the current study fetal nucleated erythrocytes were flow sorted on the basis of four parameters: cell size, cell granularity, transferrin receptor, and glycophorin-A cell surface molecule. By polymerase chain reaction with oligonucleotide primers flanking single-copy Y-specific deoxyribonucleic acid sequences, male fetuses were correctly identified among flow-sorted samples in 12 of 12 (100%) pregnancies; female fetuses were correctly identified in 5 of 6 (83%) pregnancies. We also achieved the prenatal diagnosis of fetal aneuploidies by use of flow-sorted nucleated fetal erythrocytes and in situ hybridization with chromosome-specific deoxyribonucleic acid probes: one case of trisomy 21 that was oetected in maternal blood taken 1 week after chorionic villus sampling and one case of trisomy 18 that was detected in maternal blood taken immediately before chrionic villus sampling. Although our results are promising, additional data on the background sensitivity and specificity of in situ hybridization in flow-sorted fetal cells will be necessary to minimize subjective interpretation and permit clinical application.

Original languageEnglish (US)
Pages (from-to)1731-1737
Number of pages7
JournalAmerican Journal of Obstetrics and Gynecology
Volume165
Issue number6 PART 1
DOIs
StatePublished - Jan 1 1991

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Prenatal Diagnosis
Flow Cytometry
Mothers
Erythroblasts
In Situ Hybridization
Fetus
Chorionic Villi Sampling
Glycophorin
Pregnancy
Transferrin Receptors
DNA Primers
DNA
Medical Genetics
Aneuploidy
Down Syndrome
Cell Size
Cytogenetics
Pregnant Women
Chromosomes
Sensitivity and Specificity

All Science Journal Classification (ASJC) codes

  • Obstetrics and Gynecology

Cite this

Price, J. O., Elias, S., Wachtel, S. S., Klinger, K., Dockter, M., Tharapel, A., ... Simpson, J. L. (1991). Prenatal diagnosis with fetal cells isolated from maternal blood by multiparameter flow cytometry. American Journal of Obstetrics and Gynecology, 165(6 PART 1), 1731-1737. https://doi.org/10.1016/0002-9378(91)90024-L

Prenatal diagnosis with fetal cells isolated from maternal blood by multiparameter flow cytometry. / Price, James O.; Elias, Sherman; Wachtel, Stephen S.; Klinger, Katherine; Dockter, Michael; Tharapel, Avirachan; Shulman, Lee P.; Phillips, Owen; Meyers, Carole M.; Shook, Donna; Simpson, Joe Leigh.

In: American Journal of Obstetrics and Gynecology, Vol. 165, No. 6 PART 1, 01.01.1991, p. 1731-1737.

Research output: Contribution to journalArticle

Price, JO, Elias, S, Wachtel, SS, Klinger, K, Dockter, M, Tharapel, A, Shulman, LP, Phillips, O, Meyers, CM, Shook, D & Simpson, JL 1991, 'Prenatal diagnosis with fetal cells isolated from maternal blood by multiparameter flow cytometry', American Journal of Obstetrics and Gynecology, vol. 165, no. 6 PART 1, pp. 1731-1737. https://doi.org/10.1016/0002-9378(91)90024-L
Price, James O. ; Elias, Sherman ; Wachtel, Stephen S. ; Klinger, Katherine ; Dockter, Michael ; Tharapel, Avirachan ; Shulman, Lee P. ; Phillips, Owen ; Meyers, Carole M. ; Shook, Donna ; Simpson, Joe Leigh. / Prenatal diagnosis with fetal cells isolated from maternal blood by multiparameter flow cytometry. In: American Journal of Obstetrics and Gynecology. 1991 ; Vol. 165, No. 6 PART 1. pp. 1731-1737.
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