Preparation of acellular myocardial scaffolds with well-preserved cardiomyocyte lacunae, and method for applying mechanical and electrical simulation to tissue construct

Bo Wang, Lakiesha N. Williams, Amy Curry, Jun Liao

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

Cardiac tissue engineering/regeneration using decellularized myocardium has attracted great research attention due to its potential benefit for myocardial infarction (MI) treatment. Here we describe an optimal decellularization protocol to generate 3D porcine myocardial scaffolds with well-preserved cardiomyocyte lacunae and a multi-stimulation bioreactor that is able to provide coordinated mechanical and electrical stimulation for facilitating cardiac construct development.

Original languageEnglish (US)
Title of host publicationCardiac Tissue Engineering
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages189-202
Number of pages14
ISBN (Print)9781493910465
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1181
ISSN (Print)1064-3745

Fingerprint

Bioreactors
Tissue Engineering
Cardiac Myocytes
Electric Stimulation
Regeneration
Myocardium
Swine
Myocardial Infarction
Research

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Wang, B., Williams, L. N., Curry, A., & Liao, J. (2014). Preparation of acellular myocardial scaffolds with well-preserved cardiomyocyte lacunae, and method for applying mechanical and electrical simulation to tissue construct. In Cardiac Tissue Engineering: Methods and Protocols (pp. 189-202). (Methods in Molecular Biology; Vol. 1181). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-1047-2_17

Preparation of acellular myocardial scaffolds with well-preserved cardiomyocyte lacunae, and method for applying mechanical and electrical simulation to tissue construct. / Wang, Bo; Williams, Lakiesha N.; Curry, Amy; Liao, Jun.

Cardiac Tissue Engineering: Methods and Protocols. Humana Press Inc., 2014. p. 189-202 (Methods in Molecular Biology; Vol. 1181).

Research output: Chapter in Book/Report/Conference proceedingChapter

Wang, B, Williams, LN, Curry, A & Liao, J 2014, Preparation of acellular myocardial scaffolds with well-preserved cardiomyocyte lacunae, and method for applying mechanical and electrical simulation to tissue construct. in Cardiac Tissue Engineering: Methods and Protocols. Methods in Molecular Biology, vol. 1181, Humana Press Inc., pp. 189-202. https://doi.org/10.1007/978-1-4939-1047-2_17
Wang B, Williams LN, Curry A, Liao J. Preparation of acellular myocardial scaffolds with well-preserved cardiomyocyte lacunae, and method for applying mechanical and electrical simulation to tissue construct. In Cardiac Tissue Engineering: Methods and Protocols. Humana Press Inc. 2014. p. 189-202. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-1047-2_17
Wang, Bo ; Williams, Lakiesha N. ; Curry, Amy ; Liao, Jun. / Preparation of acellular myocardial scaffolds with well-preserved cardiomyocyte lacunae, and method for applying mechanical and electrical simulation to tissue construct. Cardiac Tissue Engineering: Methods and Protocols. Humana Press Inc., 2014. pp. 189-202 (Methods in Molecular Biology).
@inbook{d1cf9641c6b44d2a8a4f078536e4bf04,
title = "Preparation of acellular myocardial scaffolds with well-preserved cardiomyocyte lacunae, and method for applying mechanical and electrical simulation to tissue construct",
abstract = "Cardiac tissue engineering/regeneration using decellularized myocardium has attracted great research attention due to its potential benefit for myocardial infarction (MI) treatment. Here we describe an optimal decellularization protocol to generate 3D porcine myocardial scaffolds with well-preserved cardiomyocyte lacunae and a multi-stimulation bioreactor that is able to provide coordinated mechanical and electrical stimulation for facilitating cardiac construct development.",
author = "Bo Wang and Williams, {Lakiesha N.} and Amy Curry and Jun Liao",
year = "2014",
month = "1",
day = "1",
doi = "10.1007/978-1-4939-1047-2_17",
language = "English (US)",
isbn = "9781493910465",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "189--202",
booktitle = "Cardiac Tissue Engineering",

}

TY - CHAP

T1 - Preparation of acellular myocardial scaffolds with well-preserved cardiomyocyte lacunae, and method for applying mechanical and electrical simulation to tissue construct

AU - Wang, Bo

AU - Williams, Lakiesha N.

AU - Curry, Amy

AU - Liao, Jun

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Cardiac tissue engineering/regeneration using decellularized myocardium has attracted great research attention due to its potential benefit for myocardial infarction (MI) treatment. Here we describe an optimal decellularization protocol to generate 3D porcine myocardial scaffolds with well-preserved cardiomyocyte lacunae and a multi-stimulation bioreactor that is able to provide coordinated mechanical and electrical stimulation for facilitating cardiac construct development.

AB - Cardiac tissue engineering/regeneration using decellularized myocardium has attracted great research attention due to its potential benefit for myocardial infarction (MI) treatment. Here we describe an optimal decellularization protocol to generate 3D porcine myocardial scaffolds with well-preserved cardiomyocyte lacunae and a multi-stimulation bioreactor that is able to provide coordinated mechanical and electrical stimulation for facilitating cardiac construct development.

UR - http://www.scopus.com/inward/record.url?scp=84925883854&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84925883854&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-1047-2_17

DO - 10.1007/978-1-4939-1047-2_17

M3 - Chapter

C2 - 25070338

AN - SCOPUS:84925883854

SN - 9781493910465

T3 - Methods in Molecular Biology

SP - 189

EP - 202

BT - Cardiac Tissue Engineering

PB - Humana Press Inc.

ER -