Presence of antibodies to sperm YLP12 synthetic peptide in sera and seminal plasma of immunoinfertile men

R. K. Naz, Subhash Chauhan

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Using an enzyme-linked immunosorbent assay (ELISA), sera (n = 15) and seminal plasma (n = 30) from antisperm antibody-positive immunoinfertile men (n = 45) and from fertile men (n = 45), were tested for the immunoreactivity with the synthetic YLP12 sperm peptide. Of the 15 immunoinfertile sera tested, 46% were positive for immunoglobulin (Ig)M, 73% for IgG, and 40% for IgA. Of the 30 samples of immunoinfertile seminal plasma tested, 10% were positive for IgM, 20% for IgG, and 43% for IgA. None of the sera or seminal plasma from fertile men showed a positive reaction. There was no significant correlation between the sperm immobilization technique (SIT) or tray agglutination technique (TAT) titres or percentage binding in immunobead binding technique (IBT) and the antibody reactivity for any class in the ELISA. The YLP12 peptide conjugated to bovine serum albumin-Sepharose 4B beads pulled out IgG antibodies from the serum of the immunoinfertile, but not the fertile, men. The beads pulled out IgA antibodies from the immunoinfertile, but not the fertile, seminal plasma. The immuno-affinity purified antipeptide antibodies reacted with a specific band of 72 ± 5 kDa in the human testis and with a specific band of ∼50 ± 5 kDa in the human sperm extracts. The YLP12 peptide may have applications in the specific diagnosis and treatment of male infertility and in contraceptive vaccine development.

Original languageEnglish (US)
Pages (from-to)21-26
Number of pages6
JournalMolecular Human Reproduction
Volume7
Issue number1
StatePublished - Jan 1 2001
Externally publishedYes

Fingerprint

Semen
Spermatozoa
Peptides
Antibodies
Immunoglobulin A
Serum
Immunoglobulin G
Immunoglobulin M
Contraceptive Vaccines
Enzyme-Linked Immunosorbent Assay
Male Infertility
Agglutination
Bovine Serum Albumin
Immobilization
Sepharose
Testis
Therapeutics

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Embryology
  • Molecular Biology
  • Genetics
  • Obstetrics and Gynecology
  • Developmental Biology
  • Cell Biology

Cite this

Presence of antibodies to sperm YLP12 synthetic peptide in sera and seminal plasma of immunoinfertile men. / Naz, R. K.; Chauhan, Subhash.

In: Molecular Human Reproduction, Vol. 7, No. 1, 01.01.2001, p. 21-26.

Research output: Contribution to journalArticle

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