Preservation of competent rabbit lung function after 30 hours of storage with a low-potassium dextran solution

K. Moriyasu, Peter Mckeown, D. Novitzky, T. R. Snow

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: The goal of organ preservation is maintenance of physiologic function during extended extracorporeal storage. Methods: This study was designed to evaluate the efficacy of using low-potassium (4 mmol/L) dextran (1%) solution on lung function after 30 hours hypothermic (10° C) storage and to compare this with lung function after no storage. With low-potassium dextran solution rabbit lungs were flushed (10° C, 40 ml/kg, 60 cm H2O), excised, inflated (with room air), and either not stored (control; no preservation; n = 9) or stored in low-potassium dextran solution (10° C) 30 hours (experimental group; n = 9). Results: During the flush the infusion pressure and pulmonary vascular resistance for the two groups did not differ (17.56 ± 1.3 versus 16.74 ± 1.5 mm Hg/ml/sec). After either no preservation or after 30 hours of storage, the lungs were first reperfused with low- potassium dextran solution (37° C) for 4 minutes and then with blood (37° C) for 30 minutes at 100 ml/min. During the reperfusion period the mean pulmonary artery pressure and end-inspiratory airway pressure for the control and experimental groups did not differ. After reperfusion the wet and dry weights of the left lung were determined. The wet/dry ratio for the two groups did not differ (5.32 ± 2.20 versus 4.70 ± 2.70, respectively). Conclusions: These data suggest thai cold flush, cold storage, and initial warm perfusion with low-potassium dextran solution crystalloid preserve lung function after 30 hours of storage.

Original languageEnglish (US)
Pages (from-to)75-79
Number of pages5
JournalJournal of Heart and Lung Transplantation
Volume14
Issue number1 I
StatePublished - Jan 1 1995
Externally publishedYes

Fingerprint

Dextrans
Potassium
Rabbits
Lung
Pressure
Reperfusion
Organ Preservation
Airway Management
Vascular Resistance
Pulmonary Artery
Perfusion
Air
Maintenance
Weights and Measures
Control Groups

All Science Journal Classification (ASJC) codes

  • Surgery
  • Pulmonary and Respiratory Medicine
  • Cardiology and Cardiovascular Medicine
  • Transplantation

Cite this

Preservation of competent rabbit lung function after 30 hours of storage with a low-potassium dextran solution. / Moriyasu, K.; Mckeown, Peter; Novitzky, D.; Snow, T. R.

In: Journal of Heart and Lung Transplantation, Vol. 14, No. 1 I, 01.01.1995, p. 75-79.

Research output: Contribution to journalArticle

@article{570036d8c9c84c1abe26bfd2a514eb34,
title = "Preservation of competent rabbit lung function after 30 hours of storage with a low-potassium dextran solution",
abstract = "Background: The goal of organ preservation is maintenance of physiologic function during extended extracorporeal storage. Methods: This study was designed to evaluate the efficacy of using low-potassium (4 mmol/L) dextran (1{\%}) solution on lung function after 30 hours hypothermic (10° C) storage and to compare this with lung function after no storage. With low-potassium dextran solution rabbit lungs were flushed (10° C, 40 ml/kg, 60 cm H2O), excised, inflated (with room air), and either not stored (control; no preservation; n = 9) or stored in low-potassium dextran solution (10° C) 30 hours (experimental group; n = 9). Results: During the flush the infusion pressure and pulmonary vascular resistance for the two groups did not differ (17.56 ± 1.3 versus 16.74 ± 1.5 mm Hg/ml/sec). After either no preservation or after 30 hours of storage, the lungs were first reperfused with low- potassium dextran solution (37° C) for 4 minutes and then with blood (37° C) for 30 minutes at 100 ml/min. During the reperfusion period the mean pulmonary artery pressure and end-inspiratory airway pressure for the control and experimental groups did not differ. After reperfusion the wet and dry weights of the left lung were determined. The wet/dry ratio for the two groups did not differ (5.32 ± 2.20 versus 4.70 ± 2.70, respectively). Conclusions: These data suggest thai cold flush, cold storage, and initial warm perfusion with low-potassium dextran solution crystalloid preserve lung function after 30 hours of storage.",
author = "K. Moriyasu and Peter Mckeown and D. Novitzky and Snow, {T. R.}",
year = "1995",
month = "1",
day = "1",
language = "English (US)",
volume = "14",
pages = "75--79",
journal = "Journal of Heart and Lung Transplantation",
issn = "1053-2498",
publisher = "Elsevier USA",
number = "1 I",

}

TY - JOUR

T1 - Preservation of competent rabbit lung function after 30 hours of storage with a low-potassium dextran solution

AU - Moriyasu, K.

AU - Mckeown, Peter

AU - Novitzky, D.

AU - Snow, T. R.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - Background: The goal of organ preservation is maintenance of physiologic function during extended extracorporeal storage. Methods: This study was designed to evaluate the efficacy of using low-potassium (4 mmol/L) dextran (1%) solution on lung function after 30 hours hypothermic (10° C) storage and to compare this with lung function after no storage. With low-potassium dextran solution rabbit lungs were flushed (10° C, 40 ml/kg, 60 cm H2O), excised, inflated (with room air), and either not stored (control; no preservation; n = 9) or stored in low-potassium dextran solution (10° C) 30 hours (experimental group; n = 9). Results: During the flush the infusion pressure and pulmonary vascular resistance for the two groups did not differ (17.56 ± 1.3 versus 16.74 ± 1.5 mm Hg/ml/sec). After either no preservation or after 30 hours of storage, the lungs were first reperfused with low- potassium dextran solution (37° C) for 4 minutes and then with blood (37° C) for 30 minutes at 100 ml/min. During the reperfusion period the mean pulmonary artery pressure and end-inspiratory airway pressure for the control and experimental groups did not differ. After reperfusion the wet and dry weights of the left lung were determined. The wet/dry ratio for the two groups did not differ (5.32 ± 2.20 versus 4.70 ± 2.70, respectively). Conclusions: These data suggest thai cold flush, cold storage, and initial warm perfusion with low-potassium dextran solution crystalloid preserve lung function after 30 hours of storage.

AB - Background: The goal of organ preservation is maintenance of physiologic function during extended extracorporeal storage. Methods: This study was designed to evaluate the efficacy of using low-potassium (4 mmol/L) dextran (1%) solution on lung function after 30 hours hypothermic (10° C) storage and to compare this with lung function after no storage. With low-potassium dextran solution rabbit lungs were flushed (10° C, 40 ml/kg, 60 cm H2O), excised, inflated (with room air), and either not stored (control; no preservation; n = 9) or stored in low-potassium dextran solution (10° C) 30 hours (experimental group; n = 9). Results: During the flush the infusion pressure and pulmonary vascular resistance for the two groups did not differ (17.56 ± 1.3 versus 16.74 ± 1.5 mm Hg/ml/sec). After either no preservation or after 30 hours of storage, the lungs were first reperfused with low- potassium dextran solution (37° C) for 4 minutes and then with blood (37° C) for 30 minutes at 100 ml/min. During the reperfusion period the mean pulmonary artery pressure and end-inspiratory airway pressure for the control and experimental groups did not differ. After reperfusion the wet and dry weights of the left lung were determined. The wet/dry ratio for the two groups did not differ (5.32 ± 2.20 versus 4.70 ± 2.70, respectively). Conclusions: These data suggest thai cold flush, cold storage, and initial warm perfusion with low-potassium dextran solution crystalloid preserve lung function after 30 hours of storage.

UR - http://www.scopus.com/inward/record.url?scp=0028931959&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028931959&partnerID=8YFLogxK

M3 - Article

VL - 14

SP - 75

EP - 79

JO - Journal of Heart and Lung Transplantation

JF - Journal of Heart and Lung Transplantation

SN - 1053-2498

IS - 1 I

ER -