Preservation of function and histologic appearance in the injured glottis with topical mitomycin-C

Jared E. Spector, Jay A. Werkhaven, Nicole C. Spector, Shan Huang, Robert Page, Bryan Baranowski, Matthew Luther, Bartley McGehee, Lou Reinisch

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Objective: To evaluate the functional and histological effects of a single application of topical mitomycin-C after laser injury in the posterior canine glottis. Study Design: A prospective, randomized study of 16 canines. Methods: A supersaturated (1%) solution of topical mitomycin-C was applied to a unilateral, laser-induced injury near the cricoarytenoid joint in eight dogs. The mitomycin-soaked pledget was placed immediately after induction of the injury and was left in contact with exposed cartilage for 3 minutes. The opposite side was not injured to provide an internal control. In eight additional dogs, the same laser injury was allowed to heal untreated. After 6 weeks, the animals were sacrificed and their larynges harvested. Arytenoid adduction sutures were placed bilaterally, and the force required to bring the vocal folds to midline was measured for each side using tensiometry. Gross and microscopic histological analysis was performed. Statistical analysis was accomplished using a two-tailed Student t test of unpaired samples, and the Wilcoxon Signed Rank Test where appropriate. Results: The mitomycin-C treated larynges demonstrated improved cricoarytenoid joint mobility (P =. 007), decreased granulation tissue development (P =. 03), and complete prevention of secondary "vocal granuloma" formation (P =. 0004) when compared with eight dogs with identical laser injuries allowed to heal untreated. No complications were noted. Conclusions: This study demonstrates functional preservation and improved histological appearance of the injured glottis after a single treatment with topical mitomycin-C. Potential applications of these findings include prophylactic use of topical mitomycin-C on glottic insults that commonly progress from granulation tissue formation to scarring and decreased vocal fold function.

Original languageEnglish (US)
Pages (from-to)1125-1129
Number of pages5
JournalLaryngoscope
Volume109
Issue number7
DOIs
StatePublished - Jan 1 1999
Externally publishedYes

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Glottis
Mitomycin
Lasers
Wounds and Injuries
Granulation Tissue
Vocal Cords
Dogs
Larynx
Canidae
Joints
Nonparametric Statistics
Secondary Prevention
Granuloma
Tongue
Sutures
Cartilage
Cicatrix
Prospective Studies
Students

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology

Cite this

Spector, J. E., Werkhaven, J. A., Spector, N. C., Huang, S., Page, R., Baranowski, B., ... Reinisch, L. (1999). Preservation of function and histologic appearance in the injured glottis with topical mitomycin-C. Laryngoscope, 109(7), 1125-1129. https://doi.org/10.1097/00005537-199907000-00022

Preservation of function and histologic appearance in the injured glottis with topical mitomycin-C. / Spector, Jared E.; Werkhaven, Jay A.; Spector, Nicole C.; Huang, Shan; Page, Robert; Baranowski, Bryan; Luther, Matthew; McGehee, Bartley; Reinisch, Lou.

In: Laryngoscope, Vol. 109, No. 7, 01.01.1999, p. 1125-1129.

Research output: Contribution to journalArticle

Spector, JE, Werkhaven, JA, Spector, NC, Huang, S, Page, R, Baranowski, B, Luther, M, McGehee, B & Reinisch, L 1999, 'Preservation of function and histologic appearance in the injured glottis with topical mitomycin-C', Laryngoscope, vol. 109, no. 7, pp. 1125-1129. https://doi.org/10.1097/00005537-199907000-00022
Spector, Jared E. ; Werkhaven, Jay A. ; Spector, Nicole C. ; Huang, Shan ; Page, Robert ; Baranowski, Bryan ; Luther, Matthew ; McGehee, Bartley ; Reinisch, Lou. / Preservation of function and histologic appearance in the injured glottis with topical mitomycin-C. In: Laryngoscope. 1999 ; Vol. 109, No. 7. pp. 1125-1129.
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AU - Page, Robert

AU - Baranowski, Bryan

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AU - McGehee, Bartley

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N2 - Objective: To evaluate the functional and histological effects of a single application of topical mitomycin-C after laser injury in the posterior canine glottis. Study Design: A prospective, randomized study of 16 canines. Methods: A supersaturated (1%) solution of topical mitomycin-C was applied to a unilateral, laser-induced injury near the cricoarytenoid joint in eight dogs. The mitomycin-soaked pledget was placed immediately after induction of the injury and was left in contact with exposed cartilage for 3 minutes. The opposite side was not injured to provide an internal control. In eight additional dogs, the same laser injury was allowed to heal untreated. After 6 weeks, the animals were sacrificed and their larynges harvested. Arytenoid adduction sutures were placed bilaterally, and the force required to bring the vocal folds to midline was measured for each side using tensiometry. Gross and microscopic histological analysis was performed. Statistical analysis was accomplished using a two-tailed Student t test of unpaired samples, and the Wilcoxon Signed Rank Test where appropriate. Results: The mitomycin-C treated larynges demonstrated improved cricoarytenoid joint mobility (P =. 007), decreased granulation tissue development (P =. 03), and complete prevention of secondary "vocal granuloma" formation (P =. 0004) when compared with eight dogs with identical laser injuries allowed to heal untreated. No complications were noted. Conclusions: This study demonstrates functional preservation and improved histological appearance of the injured glottis after a single treatment with topical mitomycin-C. Potential applications of these findings include prophylactic use of topical mitomycin-C on glottic insults that commonly progress from granulation tissue formation to scarring and decreased vocal fold function.

AB - Objective: To evaluate the functional and histological effects of a single application of topical mitomycin-C after laser injury in the posterior canine glottis. Study Design: A prospective, randomized study of 16 canines. Methods: A supersaturated (1%) solution of topical mitomycin-C was applied to a unilateral, laser-induced injury near the cricoarytenoid joint in eight dogs. The mitomycin-soaked pledget was placed immediately after induction of the injury and was left in contact with exposed cartilage for 3 minutes. The opposite side was not injured to provide an internal control. In eight additional dogs, the same laser injury was allowed to heal untreated. After 6 weeks, the animals were sacrificed and their larynges harvested. Arytenoid adduction sutures were placed bilaterally, and the force required to bring the vocal folds to midline was measured for each side using tensiometry. Gross and microscopic histological analysis was performed. Statistical analysis was accomplished using a two-tailed Student t test of unpaired samples, and the Wilcoxon Signed Rank Test where appropriate. Results: The mitomycin-C treated larynges demonstrated improved cricoarytenoid joint mobility (P =. 007), decreased granulation tissue development (P =. 03), and complete prevention of secondary "vocal granuloma" formation (P =. 0004) when compared with eight dogs with identical laser injuries allowed to heal untreated. No complications were noted. Conclusions: This study demonstrates functional preservation and improved histological appearance of the injured glottis after a single treatment with topical mitomycin-C. Potential applications of these findings include prophylactic use of topical mitomycin-C on glottic insults that commonly progress from granulation tissue formation to scarring and decreased vocal fold function.

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