Pressure-induced leukocyte margination in lung postcapillary venules

Hideo Ichimura, Kaushik Parthasarathi, Andrew C. Issekutz, Jahar Bhattacharya

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Although pressure elevation in lung postcapillary venules increases endothelial P-selectin expression, the extent to which P-selectin causes lung leukocyte margination remains controversial. To address this issue, we optically viewed postcapillary venules of the isolated blood-perfused rat lung by real-time fluorescence imaging. To determine leukocyte margination in single postcapillary venules, we quantified the fluorescence of leukocytes labeled in situ with rhodamine 6G (R6G). Although baseline fluorescence was sparse, a 10-min pressure elevation by 10 CmH2O markedly increased R6G fluorescence. Both stopping blood flow during pressure elevation and eliminating leukocytes from the perfusion blocked the fluorescence increase, affirming that these fluorescence responses were attributable to pressure-induced leukocyte margination. A P-selectin-blocking MAb and the L- and P-selectin blocker fucoidin each inhibited the fluorescence increase, indicating that P-selectin was critical for inducing margination. Time-dependent imaging of blood-borne fluorescent beads revealed reduction of plasma velocity during pressure elevation. After pressure returned to baseline, a similar reduction of plasma velocity, established by manually decreasing the perfusion rate, prolonged margination. Our findings show that in lung postcapillary venules, the decrease in plasma velocity critically determines pressure-induced leukocyte margination.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume289
Issue number3 33-3
DOIs
StatePublished - Sep 1 2005
Externally publishedYes

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Venules
P-Selectin
Leukocytes
Fluorescence
Pressure
Lung
Perfusion
L-Selectin
Optical Imaging

All Science Journal Classification (ASJC) codes

  • Cell Biology
  • Physiology
  • Pulmonary and Respiratory Medicine

Cite this

Pressure-induced leukocyte margination in lung postcapillary venules. / Ichimura, Hideo; Parthasarathi, Kaushik; Issekutz, Andrew C.; Bhattacharya, Jahar.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 289, No. 3 33-3, 01.09.2005.

Research output: Contribution to journalArticle

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