Prevalence and morphology of leukocyte chemotactic factor 2-associated amyloid in renal biopsies

Christopher P. Larsen, Patrick D. Walker, Deborah T. Weiss, Alan Solomon

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Renal pathologists identify the protein component of renal amyloid deposits by immunohistochemistry using antibodies against known amyloidogenic proteins. The majority of amyloid cases can be categorized by a simple antibody panel that includes immunoglobulin light chains lambda and kappa, and serum amyloid A. In some instances, however, these reagents do not recognize materials that stain with Congo red or yield ambiguous staining results, thus creating a diagnostic dilemma. Chemical analysis of fibrils extracted from such a nonreactive renal biopsy led to the discovery of a previously unknown amyloid formed from leukocyte chemotactic factor 2 (LECT2). Over the past 8 years, we received 285 renal amyloid samples, of which 31 remained unclassified. In an effort to determine whether any of the latter samples were LECT2 related, tandem mass spectrometry was performed. In all, 7 of the 31 cases were identified as an amyloid LECT2 (ALECT2), a finding confirmed immunohistochemically using a LECT2-specific antibody. The deposits strongly stained for Congo red and, in most cases, had distinctive morphological features with diffuse involvement of the interstitium, arteries, and glomeruli. Hence, we believe that ALECT2 represents the third common form of renal amyloidosis.

Original languageEnglish (US)
Pages (from-to)816-819
Number of pages4
JournalKidney International
Volume77
Issue number9
DOIs
StatePublished - May 1 2010

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Chemotactic Factors
Amyloid
Leukocytes
Kidney
Biopsy
Congo Red
Antibodies
Immunoglobulin lambda-Chains
Immunoglobulin kappa-Chains
Amyloidogenic Proteins
Serum Amyloid A Protein
Amyloid Plaques
Amyloidosis
Tandem Mass Spectrometry
Coloring Agents
Arteries
Immunohistochemistry
Staining and Labeling
Proteins

All Science Journal Classification (ASJC) codes

  • Nephrology

Cite this

Prevalence and morphology of leukocyte chemotactic factor 2-associated amyloid in renal biopsies. / Larsen, Christopher P.; Walker, Patrick D.; Weiss, Deborah T.; Solomon, Alan.

In: Kidney International, Vol. 77, No. 9, 01.05.2010, p. 816-819.

Research output: Contribution to journalArticle

Larsen, Christopher P. ; Walker, Patrick D. ; Weiss, Deborah T. ; Solomon, Alan. / Prevalence and morphology of leukocyte chemotactic factor 2-associated amyloid in renal biopsies. In: Kidney International. 2010 ; Vol. 77, No. 9. pp. 816-819.
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