Prevention and treatment of DNA vaccine encoding cockroach allergen Bla g 1 in a mouse model of allergic airway inflammation

B. Zhou, M. Ensell, Y. Zhou, U. Nair, J. Glickstein, M. H. Kermany, Q. Cai, Chun Cai, W. Liu, Yunping Deng, A. Kakigi, M. Barbieri, M. Mora, S. Kanangat, T. J. Yoo

Research output: Contribution to journalArticle

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Abstract

Background: One-fourth of the US population is sensitized to the German cockroach. Primary German cockroach allergen Bla g 1 is detected in 63% of homes and 52% of childcare facilities in the United States. No effective treatment or vaccination strategies are yet available. Objectives: We evaluated the prophylactic and therapeutic efficacy of a plasmid DNA-mediated vaccination using the Bla g 1 gene in a mouse model of allergic inflammatory airway disease. Methods: A plasmid DNA vector coding for the Bla g 1 allergen controlled by cytomegalovirus promoter was constructed. To estimate the protective efficacy, BALB/c mice were given three injections of plasmid DNA-Bla g 1 prior to sensitization with two priming doses of recombinant Bla g 1 (rBla g 1) antigens, followed by nebulized rBla g 1 challenge. In the therapeutic approach, sensitization was followed by administering Bla g 1 DNA vaccine. Results: Bla g 1 vaccination significantly reduced allergen-induced airway inflammation, even after mice were presensitized and a Th2-dominant response was established. The Bla g 1 vaccination significantly reduced total inflammatory cell infiltrate, eosinophilia, secretion of Th2 cytokines IL-4 and IL-5 in bronchoalveolar lavage fluid, allergen-induced inflammatory infiltrates in the lungs, and Bla g 1-specific IgE in serum upon challenge with rBla g 1. Importantly, Bla g 1 DNA vaccination was able to induce IL-10-secreting regulatory T cells that could suppress the allergen-specific Th2 cells. Conclusion: DNA vaccination showed protective and therapeutic efficacy against a clinically relevant allergen Bla g 1.

Original languageEnglish (US)
Pages (from-to)166-174
Number of pages9
JournalAllergy: European Journal of Allergy and Clinical Immunology
Volume67
Issue number2
DOIs
StatePublished - Feb 1 2012

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Cockroaches
DNA Vaccines
Inflammation
Vaccination
Therapeutics
Blattellidae
Allergens
DNA
Plasmids
allergen Bla g 1
Th2 Cells
Interleukin-5
Bronchoalveolar Lavage Fluid
Eosinophilia
Regulatory T-Lymphocytes
Cytomegalovirus
Interleukin-4
Interleukin-10
Immunoglobulin E

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Prevention and treatment of DNA vaccine encoding cockroach allergen Bla g 1 in a mouse model of allergic airway inflammation. / Zhou, B.; Ensell, M.; Zhou, Y.; Nair, U.; Glickstein, J.; Kermany, M. H.; Cai, Q.; Cai, Chun; Liu, W.; Deng, Yunping; Kakigi, A.; Barbieri, M.; Mora, M.; Kanangat, S.; Yoo, T. J.

In: Allergy: European Journal of Allergy and Clinical Immunology, Vol. 67, No. 2, 01.02.2012, p. 166-174.

Research output: Contribution to journalArticle

Zhou, B, Ensell, M, Zhou, Y, Nair, U, Glickstein, J, Kermany, MH, Cai, Q, Cai, C, Liu, W, Deng, Y, Kakigi, A, Barbieri, M, Mora, M, Kanangat, S & Yoo, TJ 2012, 'Prevention and treatment of DNA vaccine encoding cockroach allergen Bla g 1 in a mouse model of allergic airway inflammation', Allergy: European Journal of Allergy and Clinical Immunology, vol. 67, no. 2, pp. 166-174. https://doi.org/10.1111/j.1398-9995.2011.02727.x
Zhou, B. ; Ensell, M. ; Zhou, Y. ; Nair, U. ; Glickstein, J. ; Kermany, M. H. ; Cai, Q. ; Cai, Chun ; Liu, W. ; Deng, Yunping ; Kakigi, A. ; Barbieri, M. ; Mora, M. ; Kanangat, S. ; Yoo, T. J. / Prevention and treatment of DNA vaccine encoding cockroach allergen Bla g 1 in a mouse model of allergic airway inflammation. In: Allergy: European Journal of Allergy and Clinical Immunology. 2012 ; Vol. 67, No. 2. pp. 166-174.
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abstract = "Background: One-fourth of the US population is sensitized to the German cockroach. Primary German cockroach allergen Bla g 1 is detected in 63{\%} of homes and 52{\%} of childcare facilities in the United States. No effective treatment or vaccination strategies are yet available. Objectives: We evaluated the prophylactic and therapeutic efficacy of a plasmid DNA-mediated vaccination using the Bla g 1 gene in a mouse model of allergic inflammatory airway disease. Methods: A plasmid DNA vector coding for the Bla g 1 allergen controlled by cytomegalovirus promoter was constructed. To estimate the protective efficacy, BALB/c mice were given three injections of plasmid DNA-Bla g 1 prior to sensitization with two priming doses of recombinant Bla g 1 (rBla g 1) antigens, followed by nebulized rBla g 1 challenge. In the therapeutic approach, sensitization was followed by administering Bla g 1 DNA vaccine. Results: Bla g 1 vaccination significantly reduced allergen-induced airway inflammation, even after mice were presensitized and a Th2-dominant response was established. The Bla g 1 vaccination significantly reduced total inflammatory cell infiltrate, eosinophilia, secretion of Th2 cytokines IL-4 and IL-5 in bronchoalveolar lavage fluid, allergen-induced inflammatory infiltrates in the lungs, and Bla g 1-specific IgE in serum upon challenge with rBla g 1. Importantly, Bla g 1 DNA vaccination was able to induce IL-10-secreting regulatory T cells that could suppress the allergen-specific Th2 cells. Conclusion: DNA vaccination showed protective and therapeutic efficacy against a clinically relevant allergen Bla g 1.",
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AU - Zhou, B.

AU - Ensell, M.

AU - Zhou, Y.

AU - Nair, U.

AU - Glickstein, J.

AU - Kermany, M. H.

AU - Cai, Q.

AU - Cai, Chun

AU - Liu, W.

AU - Deng, Yunping

AU - Kakigi, A.

AU - Barbieri, M.

AU - Mora, M.

AU - Kanangat, S.

AU - Yoo, T. J.

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N2 - Background: One-fourth of the US population is sensitized to the German cockroach. Primary German cockroach allergen Bla g 1 is detected in 63% of homes and 52% of childcare facilities in the United States. No effective treatment or vaccination strategies are yet available. Objectives: We evaluated the prophylactic and therapeutic efficacy of a plasmid DNA-mediated vaccination using the Bla g 1 gene in a mouse model of allergic inflammatory airway disease. Methods: A plasmid DNA vector coding for the Bla g 1 allergen controlled by cytomegalovirus promoter was constructed. To estimate the protective efficacy, BALB/c mice were given three injections of plasmid DNA-Bla g 1 prior to sensitization with two priming doses of recombinant Bla g 1 (rBla g 1) antigens, followed by nebulized rBla g 1 challenge. In the therapeutic approach, sensitization was followed by administering Bla g 1 DNA vaccine. Results: Bla g 1 vaccination significantly reduced allergen-induced airway inflammation, even after mice were presensitized and a Th2-dominant response was established. The Bla g 1 vaccination significantly reduced total inflammatory cell infiltrate, eosinophilia, secretion of Th2 cytokines IL-4 and IL-5 in bronchoalveolar lavage fluid, allergen-induced inflammatory infiltrates in the lungs, and Bla g 1-specific IgE in serum upon challenge with rBla g 1. Importantly, Bla g 1 DNA vaccination was able to induce IL-10-secreting regulatory T cells that could suppress the allergen-specific Th2 cells. Conclusion: DNA vaccination showed protective and therapeutic efficacy against a clinically relevant allergen Bla g 1.

AB - Background: One-fourth of the US population is sensitized to the German cockroach. Primary German cockroach allergen Bla g 1 is detected in 63% of homes and 52% of childcare facilities in the United States. No effective treatment or vaccination strategies are yet available. Objectives: We evaluated the prophylactic and therapeutic efficacy of a plasmid DNA-mediated vaccination using the Bla g 1 gene in a mouse model of allergic inflammatory airway disease. Methods: A plasmid DNA vector coding for the Bla g 1 allergen controlled by cytomegalovirus promoter was constructed. To estimate the protective efficacy, BALB/c mice were given three injections of plasmid DNA-Bla g 1 prior to sensitization with two priming doses of recombinant Bla g 1 (rBla g 1) antigens, followed by nebulized rBla g 1 challenge. In the therapeutic approach, sensitization was followed by administering Bla g 1 DNA vaccine. Results: Bla g 1 vaccination significantly reduced allergen-induced airway inflammation, even after mice were presensitized and a Th2-dominant response was established. The Bla g 1 vaccination significantly reduced total inflammatory cell infiltrate, eosinophilia, secretion of Th2 cytokines IL-4 and IL-5 in bronchoalveolar lavage fluid, allergen-induced inflammatory infiltrates in the lungs, and Bla g 1-specific IgE in serum upon challenge with rBla g 1. Importantly, Bla g 1 DNA vaccination was able to induce IL-10-secreting regulatory T cells that could suppress the allergen-specific Th2 cells. Conclusion: DNA vaccination showed protective and therapeutic efficacy against a clinically relevant allergen Bla g 1.

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JO - Allergy: European Journal of Allergy and Clinical Immunology

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