Probing mucin interaction behavior of magnetic nanoparticles

Vijayakumar N. Boya, Renn Lovett, Saini Setua, Vaibhav Gandhi, Prashanth Kumar Bhusetty Nagesh, Sheema Khan, Meena Jaggi, Murali Yallapu, Subhash Chauhan

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

In this study, we developed iron oxide based magnetic nanoparticles (MNPs) by precipitation of iron salts in the presence of ammonia and created four different formulations: without functionality (plain MNPs, no coating), with β-cyclodextrin (MNPs+β-CD) or pluronic 127 polymer (MNPs+F-127), and both β-cyclodextrin and pluronic 127 polymer (MNPs+β-CD-F-127) functionality for its efficient use in mucosal delivery. We studied the interaction and/or binding behavior of these MNPs formulations with porcine stomach mucin using steady-state fluorescence spectroscopy, and then quantified the bound mucin from absorption studies. Toxicity of these MNPs against cervical cancer cells and red blood cells was evaluated. Ex-vivo studies were performed using freshly collected gastrointestinal, ovarian, pancreas and colon organ tissues of pig to evaluate binding and uptake phenomenon of MNPs. Transport studies of these MNPs in mucin was evaluated using Boyden's chamber assay. All these studies together suggest that the MNPs+β-CD-F-127 formulation was strongly interacted with mucin and interestingly transported through mucin compared to other MNPs formulations. Hence, MNPs+β-CD-F-127 formulation could be a good candidate for the mucoadhesive biopharmaceuticals and drug delivery system.

Original languageEnglish (US)
Pages (from-to)258-268
Number of pages11
JournalJournal of Colloid and Interface Science
Volume488
DOIs
StatePublished - Feb 15 2017

Fingerprint

Mucins
Nanoparticles
Poloxamer
Cyclodextrins
Polymers
Cells
Fluorescence spectroscopy
Iron oxides
Ammonia
Toxicity
Assays
Blood
Iron
Salts
Tissue

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Surfaces, Coatings and Films
  • Colloid and Surface Chemistry

Cite this

Probing mucin interaction behavior of magnetic nanoparticles. / Boya, Vijayakumar N.; Lovett, Renn; Setua, Saini; Gandhi, Vaibhav; Bhusetty Nagesh, Prashanth Kumar; Khan, Sheema; Jaggi, Meena; Yallapu, Murali; Chauhan, Subhash.

In: Journal of Colloid and Interface Science, Vol. 488, 15.02.2017, p. 258-268.

Research output: Contribution to journalArticle

@article{f0bf9f060f8a47c4b3c37e6da15871b8,
title = "Probing mucin interaction behavior of magnetic nanoparticles",
abstract = "In this study, we developed iron oxide based magnetic nanoparticles (MNPs) by precipitation of iron salts in the presence of ammonia and created four different formulations: without functionality (plain MNPs, no coating), with β-cyclodextrin (MNPs+β-CD) or pluronic 127 polymer (MNPs+F-127), and both β-cyclodextrin and pluronic 127 polymer (MNPs+β-CD-F-127) functionality for its efficient use in mucosal delivery. We studied the interaction and/or binding behavior of these MNPs formulations with porcine stomach mucin using steady-state fluorescence spectroscopy, and then quantified the bound mucin from absorption studies. Toxicity of these MNPs against cervical cancer cells and red blood cells was evaluated. Ex-vivo studies were performed using freshly collected gastrointestinal, ovarian, pancreas and colon organ tissues of pig to evaluate binding and uptake phenomenon of MNPs. Transport studies of these MNPs in mucin was evaluated using Boyden's chamber assay. All these studies together suggest that the MNPs+β-CD-F-127 formulation was strongly interacted with mucin and interestingly transported through mucin compared to other MNPs formulations. Hence, MNPs+β-CD-F-127 formulation could be a good candidate for the mucoadhesive biopharmaceuticals and drug delivery system.",
author = "Boya, {Vijayakumar N.} and Renn Lovett and Saini Setua and Vaibhav Gandhi and {Bhusetty Nagesh}, {Prashanth Kumar} and Sheema Khan and Meena Jaggi and Murali Yallapu and Subhash Chauhan",
year = "2017",
month = "2",
day = "15",
doi = "10.1016/j.jcis.2016.10.090",
language = "English (US)",
volume = "488",
pages = "258--268",
journal = "Journal of Colloid and Interface Science",
issn = "0021-9797",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Probing mucin interaction behavior of magnetic nanoparticles

AU - Boya, Vijayakumar N.

AU - Lovett, Renn

AU - Setua, Saini

AU - Gandhi, Vaibhav

AU - Bhusetty Nagesh, Prashanth Kumar

AU - Khan, Sheema

AU - Jaggi, Meena

AU - Yallapu, Murali

AU - Chauhan, Subhash

PY - 2017/2/15

Y1 - 2017/2/15

N2 - In this study, we developed iron oxide based magnetic nanoparticles (MNPs) by precipitation of iron salts in the presence of ammonia and created four different formulations: without functionality (plain MNPs, no coating), with β-cyclodextrin (MNPs+β-CD) or pluronic 127 polymer (MNPs+F-127), and both β-cyclodextrin and pluronic 127 polymer (MNPs+β-CD-F-127) functionality for its efficient use in mucosal delivery. We studied the interaction and/or binding behavior of these MNPs formulations with porcine stomach mucin using steady-state fluorescence spectroscopy, and then quantified the bound mucin from absorption studies. Toxicity of these MNPs against cervical cancer cells and red blood cells was evaluated. Ex-vivo studies were performed using freshly collected gastrointestinal, ovarian, pancreas and colon organ tissues of pig to evaluate binding and uptake phenomenon of MNPs. Transport studies of these MNPs in mucin was evaluated using Boyden's chamber assay. All these studies together suggest that the MNPs+β-CD-F-127 formulation was strongly interacted with mucin and interestingly transported through mucin compared to other MNPs formulations. Hence, MNPs+β-CD-F-127 formulation could be a good candidate for the mucoadhesive biopharmaceuticals and drug delivery system.

AB - In this study, we developed iron oxide based magnetic nanoparticles (MNPs) by precipitation of iron salts in the presence of ammonia and created four different formulations: without functionality (plain MNPs, no coating), with β-cyclodextrin (MNPs+β-CD) or pluronic 127 polymer (MNPs+F-127), and both β-cyclodextrin and pluronic 127 polymer (MNPs+β-CD-F-127) functionality for its efficient use in mucosal delivery. We studied the interaction and/or binding behavior of these MNPs formulations with porcine stomach mucin using steady-state fluorescence spectroscopy, and then quantified the bound mucin from absorption studies. Toxicity of these MNPs against cervical cancer cells and red blood cells was evaluated. Ex-vivo studies were performed using freshly collected gastrointestinal, ovarian, pancreas and colon organ tissues of pig to evaluate binding and uptake phenomenon of MNPs. Transport studies of these MNPs in mucin was evaluated using Boyden's chamber assay. All these studies together suggest that the MNPs+β-CD-F-127 formulation was strongly interacted with mucin and interestingly transported through mucin compared to other MNPs formulations. Hence, MNPs+β-CD-F-127 formulation could be a good candidate for the mucoadhesive biopharmaceuticals and drug delivery system.

UR - http://www.scopus.com/inward/record.url?scp=84995379915&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84995379915&partnerID=8YFLogxK

U2 - 10.1016/j.jcis.2016.10.090

DO - 10.1016/j.jcis.2016.10.090

M3 - Article

VL - 488

SP - 258

EP - 268

JO - Journal of Colloid and Interface Science

JF - Journal of Colloid and Interface Science

SN - 0021-9797

ER -