Profiling gingival crevicular fluid from smoking and non-smoking chronic periodontitis patients.

Sidney Stein, Kevin J. Wendell, Michael Pabst, Mark Scarbecz

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

BACKGROUND: Smoking is a major risk factor for the development and progression of chronic periodontitis (CP). Gingival crevicular fluid (GCF) derived from these patients contains many proteins that could serve as important diagnostic indicators. A protein chip technology called Surface Enhanced Laser Desorption/Ionization-Time of Flight-Mass Spectrometry (SELDI-TOF-MS) has recently been developed to facilitate protein profiling of complex biological mixtures. The hypothesis to be tested was SELDI-TOF-MS could distinguish between GCF of CP patients by smoking status and pocket depth. METHODS: GCF samples collected before treatment from sixteen CP patients (eight smokers and eight non-smokers) at both shallow and deep sites were evaluated by SELDI-TOF-MS. Spectral fingerprints were constructed for both cohorts and analyzed by a two-way ANOVA according to smoking status and pocket depth. Significance threshold was set at p < 0.05. Mean molecular weight (MW) peaks and intensities were also analyzed. RESULTS: The spectral fingerprints were significantly different between the two cohorts when analyzed by ANOVA according to smoking status (p < 0.0001) but not pocket depth (p = 0.9876). Also, the mean intensity of many individual MW peaks were determined to be significantly different between the two cohorts. Several peaks ranging in MW from 11-14 kDa were only detected in the GCF obtained from smokers. CONCLUSIONS: This study has demonstrated that profiling of GCF by SELDI-TOF-MS can distinguish between CP smokers and non-smokers. Moreover, over-expressed proteins in GCF from smokers may serve as biomarkers for this high risk patient population.

Original languageEnglish (US)
Pages (from-to)20-24
Number of pages5
JournalThe Journal of the Tennessee Dental Association.
Volume86
Issue number2
StatePublished - Mar 1 2006
Externally publishedYes

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Gingival Crevicular Fluid
Chronic Periodontitis
Smoking
Mass Spectrometry
Lasers
Molecular Weight
Dermatoglyphics
Analysis of Variance
Protein Array Analysis
Proteins
Complex Mixtures
Biomarkers
Technology
Population

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Profiling gingival crevicular fluid from smoking and non-smoking chronic periodontitis patients. / Stein, Sidney; Wendell, Kevin J.; Pabst, Michael; Scarbecz, Mark.

In: The Journal of the Tennessee Dental Association., Vol. 86, No. 2, 01.03.2006, p. 20-24.

Research output: Contribution to journalArticle

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