Progenitor Cells of the Adult Human Subcortical White Matter

Neeta Roy, Martha S. Windrem, Steven A. Goldman

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Citations (Scopus)

Abstract

Oligodendrocyte progenitor cells (OPCs) seem to be extraordinarily widespread in the adult mammalian brain. The principal class of OPCs in adult rodents is a bipotential astrocyte-oligodendrocyte progenitor cell designated the O-2A progenitor, by virtue of its generation in vitro of oligodendrocytes and type 2 astrocytes, the latter comprising the traditionally recognized fibrous astrocytes of the white matter. OPCs may be specifically targeted and isolated from the white matter. This chapter outlines basic strategies for isolating OPCs from the adult white matter, using either fluorescence-activated cell sorting (FACS) or a higher-yield, less-specific alternative immunomagnetic isolation (MACS). Adult-human white-matter progenitor cells (WMPCs) give rise to multipotential neurospheres. Nonetheless, the very existence of multipotential progenitors scattered throughout the white-matter parenchyma forces one to reconsider understanding of both the nature and incidence of neural stem cells in the adult brain, and challenges the conception of the supposed rarity of adult neural progenitor and stem cells. In doing so, they point to an abundant and widespread source of cells that may be used both as a target for pharmacological induction and as a cell type appropriate for therapeutic engraftment to the diseased adult brain.

Original languageEnglish (US)
Title of host publicationMyelin Biology and Disorders
PublisherElsevier Inc.
Pages259-287
Number of pages29
Volume1
ISBN (Print)9780124395107
DOIs
StatePublished - Dec 1 2003
Externally publishedYes

Fingerprint

Oligodendroglia
Brain
Stem Cells
Stem cells
Astrocytes
Neural Stem Cells
Sorting
Fluorescence
Cells
Adult Stem Cells
Brain Diseases
White Matter
Rodentia
Flow Cytometry
Pharmacology
Incidence

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Roy, N., Windrem, M. S., & Goldman, S. A. (2003). Progenitor Cells of the Adult Human Subcortical White Matter. In Myelin Biology and Disorders (Vol. 1, pp. 259-287). Elsevier Inc.. https://doi.org/10.1016/B978-012439510-7/50063-2

Progenitor Cells of the Adult Human Subcortical White Matter. / Roy, Neeta; Windrem, Martha S.; Goldman, Steven A.

Myelin Biology and Disorders. Vol. 1 Elsevier Inc., 2003. p. 259-287.

Research output: Chapter in Book/Report/Conference proceedingChapter

Roy, N, Windrem, MS & Goldman, SA 2003, Progenitor Cells of the Adult Human Subcortical White Matter. in Myelin Biology and Disorders. vol. 1, Elsevier Inc., pp. 259-287. https://doi.org/10.1016/B978-012439510-7/50063-2
Roy N, Windrem MS, Goldman SA. Progenitor Cells of the Adult Human Subcortical White Matter. In Myelin Biology and Disorders. Vol. 1. Elsevier Inc. 2003. p. 259-287 https://doi.org/10.1016/B978-012439510-7/50063-2
Roy, Neeta ; Windrem, Martha S. ; Goldman, Steven A. / Progenitor Cells of the Adult Human Subcortical White Matter. Myelin Biology and Disorders. Vol. 1 Elsevier Inc., 2003. pp. 259-287
@inbook{3217cf310f9e44deb3d501aeb22d14cb,
title = "Progenitor Cells of the Adult Human Subcortical White Matter",
abstract = "Oligodendrocyte progenitor cells (OPCs) seem to be extraordinarily widespread in the adult mammalian brain. The principal class of OPCs in adult rodents is a bipotential astrocyte-oligodendrocyte progenitor cell designated the O-2A progenitor, by virtue of its generation in vitro of oligodendrocytes and type 2 astrocytes, the latter comprising the traditionally recognized fibrous astrocytes of the white matter. OPCs may be specifically targeted and isolated from the white matter. This chapter outlines basic strategies for isolating OPCs from the adult white matter, using either fluorescence-activated cell sorting (FACS) or a higher-yield, less-specific alternative immunomagnetic isolation (MACS). Adult-human white-matter progenitor cells (WMPCs) give rise to multipotential neurospheres. Nonetheless, the very existence of multipotential progenitors scattered throughout the white-matter parenchyma forces one to reconsider understanding of both the nature and incidence of neural stem cells in the adult brain, and challenges the conception of the supposed rarity of adult neural progenitor and stem cells. In doing so, they point to an abundant and widespread source of cells that may be used both as a target for pharmacological induction and as a cell type appropriate for therapeutic engraftment to the diseased adult brain.",
author = "Neeta Roy and Windrem, {Martha S.} and Goldman, {Steven A.}",
year = "2003",
month = "12",
day = "1",
doi = "10.1016/B978-012439510-7/50063-2",
language = "English (US)",
isbn = "9780124395107",
volume = "1",
pages = "259--287",
booktitle = "Myelin Biology and Disorders",
publisher = "Elsevier Inc.",
address = "United States",

}

TY - CHAP

T1 - Progenitor Cells of the Adult Human Subcortical White Matter

AU - Roy, Neeta

AU - Windrem, Martha S.

AU - Goldman, Steven A.

PY - 2003/12/1

Y1 - 2003/12/1

N2 - Oligodendrocyte progenitor cells (OPCs) seem to be extraordinarily widespread in the adult mammalian brain. The principal class of OPCs in adult rodents is a bipotential astrocyte-oligodendrocyte progenitor cell designated the O-2A progenitor, by virtue of its generation in vitro of oligodendrocytes and type 2 astrocytes, the latter comprising the traditionally recognized fibrous astrocytes of the white matter. OPCs may be specifically targeted and isolated from the white matter. This chapter outlines basic strategies for isolating OPCs from the adult white matter, using either fluorescence-activated cell sorting (FACS) or a higher-yield, less-specific alternative immunomagnetic isolation (MACS). Adult-human white-matter progenitor cells (WMPCs) give rise to multipotential neurospheres. Nonetheless, the very existence of multipotential progenitors scattered throughout the white-matter parenchyma forces one to reconsider understanding of both the nature and incidence of neural stem cells in the adult brain, and challenges the conception of the supposed rarity of adult neural progenitor and stem cells. In doing so, they point to an abundant and widespread source of cells that may be used both as a target for pharmacological induction and as a cell type appropriate for therapeutic engraftment to the diseased adult brain.

AB - Oligodendrocyte progenitor cells (OPCs) seem to be extraordinarily widespread in the adult mammalian brain. The principal class of OPCs in adult rodents is a bipotential astrocyte-oligodendrocyte progenitor cell designated the O-2A progenitor, by virtue of its generation in vitro of oligodendrocytes and type 2 astrocytes, the latter comprising the traditionally recognized fibrous astrocytes of the white matter. OPCs may be specifically targeted and isolated from the white matter. This chapter outlines basic strategies for isolating OPCs from the adult white matter, using either fluorescence-activated cell sorting (FACS) or a higher-yield, less-specific alternative immunomagnetic isolation (MACS). Adult-human white-matter progenitor cells (WMPCs) give rise to multipotential neurospheres. Nonetheless, the very existence of multipotential progenitors scattered throughout the white-matter parenchyma forces one to reconsider understanding of both the nature and incidence of neural stem cells in the adult brain, and challenges the conception of the supposed rarity of adult neural progenitor and stem cells. In doing so, they point to an abundant and widespread source of cells that may be used both as a target for pharmacological induction and as a cell type appropriate for therapeutic engraftment to the diseased adult brain.

UR - http://www.scopus.com/inward/record.url?scp=84903116984&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84903116984&partnerID=8YFLogxK

U2 - 10.1016/B978-012439510-7/50063-2

DO - 10.1016/B978-012439510-7/50063-2

M3 - Chapter

SN - 9780124395107

VL - 1

SP - 259

EP - 287

BT - Myelin Biology and Disorders

PB - Elsevier Inc.

ER -