Progesterone reduces lipopolysaccharide induced interleukin-6 secretion in fetoplacental chorionic arteries, fractionated cord blood, and maternal mononuclear cells

Jennifer L. Gotkin, Jeremy Celver, Patrick McNutt, Andrea D. Shields, Bobby Howard, Damian J. Paonessa, Peter G. Napolitano

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Objective: The purpose of this study was to characterize effect of progesterone (P4) on interleukin-6 (IL-6) production by fetoplacental artery explants, fetal granulocytes, and fetal and maternal mononuclear cells. Study design: Arteries and cord blood were obtained from 5 term pregnancies undergoing repeat cesarean section. Maternal blood was obtained from another 6 women at 16 to 20 weeks' gestation. Tissues were fractionated by dissection or Histopaque gradient. Specimens were incubated in physiologic media then exposed to lipopolysaccharide (LPS) or P4 alone, or pretreated with P4 and then exposed to LPS. Samples were evaluated for IL-6 by enzyme-linked immunosorbent assay (ELISA). Results: Arteries and fetal and maternal mononuclear cells exposed to LPS increased IL-6 secretion by 9-, 27-, and 29-fold, respectively. P4 pretreatment blocked LPS induction of IL-6. Fetal granulocytes did not increase IL-6 production in response to LPS exposure. Conclusion: LPS induces IL-6 in arteries and fetal and maternal mononuclear cells. P4 pretreatment significantly blocks this effect in these cell populations, suggesting possible targets for anti-inflammatory actions of P4 in prevention of preterm birth.

Original languageEnglish (US)
Pages (from-to)1015-1019
Number of pages5
JournalAmerican Journal of Obstetrics and Gynecology
Volume195
Issue number4
DOIs
StatePublished - Oct 1 2006
Externally publishedYes

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Fetal Blood
Progesterone
Lipopolysaccharides
Interleukin-6
Arteries
Mothers
Granulocytes
Repeat Cesarean Section
Pregnancy
Premature Birth
Dissection
Anti-Inflammatory Agents
Enzyme-Linked Immunosorbent Assay
Population

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

Progesterone reduces lipopolysaccharide induced interleukin-6 secretion in fetoplacental chorionic arteries, fractionated cord blood, and maternal mononuclear cells. / Gotkin, Jennifer L.; Celver, Jeremy; McNutt, Patrick; Shields, Andrea D.; Howard, Bobby; Paonessa, Damian J.; Napolitano, Peter G.

In: American Journal of Obstetrics and Gynecology, Vol. 195, No. 4, 01.10.2006, p. 1015-1019.

Research output: Contribution to journalArticle

Gotkin, Jennifer L. ; Celver, Jeremy ; McNutt, Patrick ; Shields, Andrea D. ; Howard, Bobby ; Paonessa, Damian J. ; Napolitano, Peter G. / Progesterone reduces lipopolysaccharide induced interleukin-6 secretion in fetoplacental chorionic arteries, fractionated cord blood, and maternal mononuclear cells. In: American Journal of Obstetrics and Gynecology. 2006 ; Vol. 195, No. 4. pp. 1015-1019.
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