Prognosis test by visualization of demineralized dentin under restorations to prevent initial wall-lesions initiated by lactic acid

Morakot Piemjai, Pareewan Chantarawej, Nobuo Nakabayashi, Franklin Garcia-Godoy

Research output: Contribution to journalArticle

Abstract

Purpose: To visualize the complete protection of restored dentin in the acidic environment by the complete impregnation of resin into demineralized dentin. Methods: Class V cavities prepared in extracted human molars composed of enamel, cementum and dentin surfaces were sealed using either 4-META/MMA-TBB resin with conditioning periods of 10, 30, 60 seconds with 10% citric acid and 3% ferric chloride mixed aqueous solution (10-3) or Single-Bond 2 adhesives prior to restoration with light-cured resin-composite. Specimens were either immersed in artificial saliva or 0.1 mol/L buffered lactic acid solutions at 37°C for 14 days. After immersion in 0.5% basic fuchsin dye for 24 hours, the extent of dye penetration at the margin was measured. SEM micrography was used to investigate the quality of hybridized dentin when immersed in HCl followed by NaOCl solutions. Results: After lactic acid exposure, dye penetration into the adjacent demineralized cementum/dentin was found to be significantly lower than the leakage distance under 30 and 60 seconds and Single-Bond 2 specimens. Leakage-free margins were found in 10-second conditioning period specimens for both control and after soaking in buffered lactic solution with the stable hybridized dentin after chemical challenge.

Original languageEnglish (US)
Pages (from-to)119-124
Number of pages6
JournalAmerican Journal of Dentistry
Volume30
Issue number3
StatePublished - Jun 1 2017

Fingerprint

Dentin
Lactic Acid
Dental Cementum
Coloring Agents
Artificial Saliva
Composite Resins
Immersion
Dental Enamel
Citric Acid
Adhesives
Milk
Light

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Prognosis test by visualization of demineralized dentin under restorations to prevent initial wall-lesions initiated by lactic acid. / Piemjai, Morakot; Chantarawej, Pareewan; Nakabayashi, Nobuo; Garcia-Godoy, Franklin.

In: American Journal of Dentistry, Vol. 30, No. 3, 01.06.2017, p. 119-124.

Research output: Contribution to journalArticle

@article{934656daa6644e91989f8d5a899e663c,
title = "Prognosis test by visualization of demineralized dentin under restorations to prevent initial wall-lesions initiated by lactic acid",
abstract = "Purpose: To visualize the complete protection of restored dentin in the acidic environment by the complete impregnation of resin into demineralized dentin. Methods: Class V cavities prepared in extracted human molars composed of enamel, cementum and dentin surfaces were sealed using either 4-META/MMA-TBB resin with conditioning periods of 10, 30, 60 seconds with 10{\%} citric acid and 3{\%} ferric chloride mixed aqueous solution (10-3) or Single-Bond 2 adhesives prior to restoration with light-cured resin-composite. Specimens were either immersed in artificial saliva or 0.1 mol/L buffered lactic acid solutions at 37°C for 14 days. After immersion in 0.5{\%} basic fuchsin dye for 24 hours, the extent of dye penetration at the margin was measured. SEM micrography was used to investigate the quality of hybridized dentin when immersed in HCl followed by NaOCl solutions. Results: After lactic acid exposure, dye penetration into the adjacent demineralized cementum/dentin was found to be significantly lower than the leakage distance under 30 and 60 seconds and Single-Bond 2 specimens. Leakage-free margins were found in 10-second conditioning period specimens for both control and after soaking in buffered lactic solution with the stable hybridized dentin after chemical challenge.",
author = "Morakot Piemjai and Pareewan Chantarawej and Nobuo Nakabayashi and Franklin Garcia-Godoy",
year = "2017",
month = "6",
day = "1",
language = "English (US)",
volume = "30",
pages = "119--124",
journal = "American Journal of Dentistry",
issn = "0894-8275",
publisher = "Mosher and Linder, Inc",
number = "3",

}

TY - JOUR

T1 - Prognosis test by visualization of demineralized dentin under restorations to prevent initial wall-lesions initiated by lactic acid

AU - Piemjai, Morakot

AU - Chantarawej, Pareewan

AU - Nakabayashi, Nobuo

AU - Garcia-Godoy, Franklin

PY - 2017/6/1

Y1 - 2017/6/1

N2 - Purpose: To visualize the complete protection of restored dentin in the acidic environment by the complete impregnation of resin into demineralized dentin. Methods: Class V cavities prepared in extracted human molars composed of enamel, cementum and dentin surfaces were sealed using either 4-META/MMA-TBB resin with conditioning periods of 10, 30, 60 seconds with 10% citric acid and 3% ferric chloride mixed aqueous solution (10-3) or Single-Bond 2 adhesives prior to restoration with light-cured resin-composite. Specimens were either immersed in artificial saliva or 0.1 mol/L buffered lactic acid solutions at 37°C for 14 days. After immersion in 0.5% basic fuchsin dye for 24 hours, the extent of dye penetration at the margin was measured. SEM micrography was used to investigate the quality of hybridized dentin when immersed in HCl followed by NaOCl solutions. Results: After lactic acid exposure, dye penetration into the adjacent demineralized cementum/dentin was found to be significantly lower than the leakage distance under 30 and 60 seconds and Single-Bond 2 specimens. Leakage-free margins were found in 10-second conditioning period specimens for both control and after soaking in buffered lactic solution with the stable hybridized dentin after chemical challenge.

AB - Purpose: To visualize the complete protection of restored dentin in the acidic environment by the complete impregnation of resin into demineralized dentin. Methods: Class V cavities prepared in extracted human molars composed of enamel, cementum and dentin surfaces were sealed using either 4-META/MMA-TBB resin with conditioning periods of 10, 30, 60 seconds with 10% citric acid and 3% ferric chloride mixed aqueous solution (10-3) or Single-Bond 2 adhesives prior to restoration with light-cured resin-composite. Specimens were either immersed in artificial saliva or 0.1 mol/L buffered lactic acid solutions at 37°C for 14 days. After immersion in 0.5% basic fuchsin dye for 24 hours, the extent of dye penetration at the margin was measured. SEM micrography was used to investigate the quality of hybridized dentin when immersed in HCl followed by NaOCl solutions. Results: After lactic acid exposure, dye penetration into the adjacent demineralized cementum/dentin was found to be significantly lower than the leakage distance under 30 and 60 seconds and Single-Bond 2 specimens. Leakage-free margins were found in 10-second conditioning period specimens for both control and after soaking in buffered lactic solution with the stable hybridized dentin after chemical challenge.

UR - http://www.scopus.com/inward/record.url?scp=85021376037&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85021376037&partnerID=8YFLogxK

M3 - Article

VL - 30

SP - 119

EP - 124

JO - American Journal of Dentistry

JF - American Journal of Dentistry

SN - 0894-8275

IS - 3

ER -