Prognostic significance of p53, bcl-2 and epidermal growth factor receptor (egfr) in muscle invasive transitional cell carcinoma (tcc) of the urinary bladder

Sugandh D. Shetty, Syed K. Mohsin, James O. Peabody, Hans J. Stricker, Mahul Amin, Riad N. Farah

Research output: Contribution to journalArticle

Abstract

INTRODUCTION: The presence of p53. bcl-2 and EGFR was been evaluated in muscle invasive TCC of urinary bladder to determine their prognostic significance. METHODS: Seventy-one patients with muscle invasive TCC (10 pT2, 23 pT3a, 18 pT3b and 20 pT4) with minimum follow up of 5 years were evaluated. Representative archival sections were immunostaincd with antibodies to p53, bcl-2 and EGFR proteins using Elite ABC (Vector) technique. Due to inadequate tumor sample, staining in 8 of p53, 12 of bcl-2 and 3 of EGFR could not be done. Immunoreactivity (IR) was scored negative (<10% stained puclei) and positive (>10% stained nuclei). The relationship between pathological stage, tumor grade, vascular invasion (VIN) was correlated to the presence of each of the 3 markers. The ability of each of the markers to predict death from disease was assessed on regression analysis. RESULTS: p53, bcl-2 and EGFR expression was present in 60/63 (93%), 7/59 (12%) and 60/68 (88%) tumors respectively. Presence of p53, bcl-2, EGFR did not correlate with pathological stage, grade, VIN. However on Cox proportional regressional analysis p53 (<10% vs >10%), VTN and metastasis were predictors of death from disease ( p = 0.038. 0.017 and 0.003; Hazard ratios = 1.1, 2 and 2.4 respectively). However p53 was the strongest predictor of death from disease when a cut off of > 25% stained nuclei (Hazard ratio 2.9) was used. EGFR and bcl-2 expression were not statistically significant prognostic parameters. COCLUSIONS: In muscle invasive disease, p53 expression correlates significantly with disease specific survival.

Original languageEnglish (US)
Number of pages1
JournalBritish Journal of Urology
Volume80
Issue numberSUPPL. 2
StatePublished - Dec 1 1997
Externally publishedYes

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Transitional Cell Carcinoma
Epidermal Growth Factor Receptor
Urinary Bladder
Muscles
Blood Vessels
Neoplasms
Regression Analysis
Staining and Labeling
Neoplasm Metastasis
Antibodies
Proteins

All Science Journal Classification (ASJC) codes

  • Urology

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Prognostic significance of p53, bcl-2 and epidermal growth factor receptor (egfr) in muscle invasive transitional cell carcinoma (tcc) of the urinary bladder. / Shetty, Sugandh D.; Mohsin, Syed K.; Peabody, James O.; Stricker, Hans J.; Amin, Mahul; Farah, Riad N.

In: British Journal of Urology, Vol. 80, No. SUPPL. 2, 01.12.1997.

Research output: Contribution to journalArticle

Shetty, Sugandh D. ; Mohsin, Syed K. ; Peabody, James O. ; Stricker, Hans J. ; Amin, Mahul ; Farah, Riad N. / Prognostic significance of p53, bcl-2 and epidermal growth factor receptor (egfr) in muscle invasive transitional cell carcinoma (tcc) of the urinary bladder. In: British Journal of Urology. 1997 ; Vol. 80, No. SUPPL. 2.
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abstract = "INTRODUCTION: The presence of p53. bcl-2 and EGFR was been evaluated in muscle invasive TCC of urinary bladder to determine their prognostic significance. METHODS: Seventy-one patients with muscle invasive TCC (10 pT2, 23 pT3a, 18 pT3b and 20 pT4) with minimum follow up of 5 years were evaluated. Representative archival sections were immunostaincd with antibodies to p53, bcl-2 and EGFR proteins using Elite ABC (Vector) technique. Due to inadequate tumor sample, staining in 8 of p53, 12 of bcl-2 and 3 of EGFR could not be done. Immunoreactivity (IR) was scored negative (<10{\%} stained puclei) and positive (>10{\%} stained nuclei). The relationship between pathological stage, tumor grade, vascular invasion (VIN) was correlated to the presence of each of the 3 markers. The ability of each of the markers to predict death from disease was assessed on regression analysis. RESULTS: p53, bcl-2 and EGFR expression was present in 60/63 (93{\%}), 7/59 (12{\%}) and 60/68 (88{\%}) tumors respectively. Presence of p53, bcl-2, EGFR did not correlate with pathological stage, grade, VIN. However on Cox proportional regressional analysis p53 (<10{\%} vs >10{\%}), VTN and metastasis were predictors of death from disease ( p = 0.038. 0.017 and 0.003; Hazard ratios = 1.1, 2 and 2.4 respectively). However p53 was the strongest predictor of death from disease when a cut off of > 25{\%} stained nuclei (Hazard ratio 2.9) was used. EGFR and bcl-2 expression were not statistically significant prognostic parameters. COCLUSIONS: In muscle invasive disease, p53 expression correlates significantly with disease specific survival.",
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T1 - Prognostic significance of p53, bcl-2 and epidermal growth factor receptor (egfr) in muscle invasive transitional cell carcinoma (tcc) of the urinary bladder

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AU - Mohsin, Syed K.

AU - Peabody, James O.

AU - Stricker, Hans J.

AU - Amin, Mahul

AU - Farah, Riad N.

PY - 1997/12/1

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N2 - INTRODUCTION: The presence of p53. bcl-2 and EGFR was been evaluated in muscle invasive TCC of urinary bladder to determine their prognostic significance. METHODS: Seventy-one patients with muscle invasive TCC (10 pT2, 23 pT3a, 18 pT3b and 20 pT4) with minimum follow up of 5 years were evaluated. Representative archival sections were immunostaincd with antibodies to p53, bcl-2 and EGFR proteins using Elite ABC (Vector) technique. Due to inadequate tumor sample, staining in 8 of p53, 12 of bcl-2 and 3 of EGFR could not be done. Immunoreactivity (IR) was scored negative (<10% stained puclei) and positive (>10% stained nuclei). The relationship between pathological stage, tumor grade, vascular invasion (VIN) was correlated to the presence of each of the 3 markers. The ability of each of the markers to predict death from disease was assessed on regression analysis. RESULTS: p53, bcl-2 and EGFR expression was present in 60/63 (93%), 7/59 (12%) and 60/68 (88%) tumors respectively. Presence of p53, bcl-2, EGFR did not correlate with pathological stage, grade, VIN. However on Cox proportional regressional analysis p53 (<10% vs >10%), VTN and metastasis were predictors of death from disease ( p = 0.038. 0.017 and 0.003; Hazard ratios = 1.1, 2 and 2.4 respectively). However p53 was the strongest predictor of death from disease when a cut off of > 25% stained nuclei (Hazard ratio 2.9) was used. EGFR and bcl-2 expression were not statistically significant prognostic parameters. COCLUSIONS: In muscle invasive disease, p53 expression correlates significantly with disease specific survival.

AB - INTRODUCTION: The presence of p53. bcl-2 and EGFR was been evaluated in muscle invasive TCC of urinary bladder to determine their prognostic significance. METHODS: Seventy-one patients with muscle invasive TCC (10 pT2, 23 pT3a, 18 pT3b and 20 pT4) with minimum follow up of 5 years were evaluated. Representative archival sections were immunostaincd with antibodies to p53, bcl-2 and EGFR proteins using Elite ABC (Vector) technique. Due to inadequate tumor sample, staining in 8 of p53, 12 of bcl-2 and 3 of EGFR could not be done. Immunoreactivity (IR) was scored negative (<10% stained puclei) and positive (>10% stained nuclei). The relationship between pathological stage, tumor grade, vascular invasion (VIN) was correlated to the presence of each of the 3 markers. The ability of each of the markers to predict death from disease was assessed on regression analysis. RESULTS: p53, bcl-2 and EGFR expression was present in 60/63 (93%), 7/59 (12%) and 60/68 (88%) tumors respectively. Presence of p53, bcl-2, EGFR did not correlate with pathological stage, grade, VIN. However on Cox proportional regressional analysis p53 (<10% vs >10%), VTN and metastasis were predictors of death from disease ( p = 0.038. 0.017 and 0.003; Hazard ratios = 1.1, 2 and 2.4 respectively). However p53 was the strongest predictor of death from disease when a cut off of > 25% stained nuclei (Hazard ratio 2.9) was used. EGFR and bcl-2 expression were not statistically significant prognostic parameters. COCLUSIONS: In muscle invasive disease, p53 expression correlates significantly with disease specific survival.

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