Prolactin binding in the developing rat fetal liver

Ramasubbareddy Dhanireddy, Rodney E. Ulane

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The binding of prlactin by fetal rat liver cell membrane fractions from 17 to 21 days gestation was studied. Particulate liver membranes were prepared in Dulbecco's Phosphate Buffered Saline (PBS) by ultracentrifugation and incubated at 22° C for 16 hours with [125I] iodo-human growth hormone (hGH). Non-specific binding was assessed by parallel incubations in the presence of a 2000-fold excess ovine prolactin. Specific prolactin binding sites were detected only at 21 days gestation (2932 ± 401 cpm/mg protein) in freshly prepared membranes. On freezing at -20°C for 24 to 48 hours, the membranes of 20 days gestation animals were able to specifically bind prolactin (1295 ± 239 cpm/mg protein). Freezing led to a 45 ± 7% increase (4270 ± 701 cpm/mg protein) in prolactin binding at 21 days gestation. No hormonal binding was detected from 17 through 19 days gestation in either fresh or freeze-thawed membranes. Scatchard analysis revealed a high affinity binding site with a Ka of approximately 1.4 × 108M-1 in both fresh and freeze-thawed membrane preparations. The data show that 1) prolactin receptors appear in liver only during late fetal life and that 2) freezing of membranes may unmask binding sites that are initially unavailable to specifically bind prolactin.

Original languageEnglish (US)
Pages (from-to)733-740
Number of pages8
JournalLife Sciences
Volume35
Issue number7
DOIs
StatePublished - Aug 13 1984
Externally publishedYes

Fingerprint

Prolactin
Liver
Rats
Membranes
Pregnancy
Freezing
Binding Sites
Prolactin Receptors
Proteins
Human Growth Hormone
Ultracentrifugation
Cell membranes
Sheep
Animals
Phosphates
Cell Membrane

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Prolactin binding in the developing rat fetal liver. / Dhanireddy, Ramasubbareddy; Ulane, Rodney E.

In: Life Sciences, Vol. 35, No. 7, 13.08.1984, p. 733-740.

Research output: Contribution to journalArticle

Dhanireddy, Ramasubbareddy ; Ulane, Rodney E. / Prolactin binding in the developing rat fetal liver. In: Life Sciences. 1984 ; Vol. 35, No. 7. pp. 733-740.
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