Proliferation of astroglia from the adult human cerebrum is inhibited by ethanol in vitro

Cynthia J.M. Kane, April Berry, Frederick Boop, David L. Davies

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Chronic alcoholism is associated with atrophy of the adult brain, while fetal exposure to ethanol can cause microencephaly. Since astroglial pathology is a common feature of ethanol exposure in both humans and animal models, the direct influence of ethanol on proliferation of human astroglia from the gray and white matter of adult temporal lobe was determined and compared. Astroglial cultures were exposed to constant concentrations of ethanol at realistic social and clinical levels (0.1, 0.2 or 0.5%; w/v) for 1 to 5 days. Proliferation was quantified by bromodeoxyuridine labeling and enumeration of replicating cells. Ethanol exposure significantly inhibited proliferation of both gray and white matter astroglia in a dose and duration dependent manner. Gray matter was slightly more sensitive than white matter to inhibition by low to moderate concentrations of ethanol; in contrast, white matter was more sensitive to high ethanol concentrations. Maximum inhibition was 20% in gray matter and 25% in white matter. Human astroglial proliferation was directly inhibited in the absence of neurons, microglia, neuronal degeneration or systemic factors that have confounded in vivo studies. Restricted astroglial proliferation may underlie aspects of the astroglial pathology associated with ethanol exposure.

Original languageEnglish (US)
Pages (from-to)39-44
Number of pages6
JournalBrain Research
Volume731
Issue number1-2
DOIs
StatePublished - Aug 26 1996

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Cerebrum
Astrocytes
Ethanol
Pathology
In Vitro Techniques
Microglia
Temporal Lobe
Bromodeoxyuridine
Alcoholism
Atrophy
Animal Models
White Matter
Neurons
Gray Matter
Brain

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Proliferation of astroglia from the adult human cerebrum is inhibited by ethanol in vitro. / Kane, Cynthia J.M.; Berry, April; Boop, Frederick; Davies, David L.

In: Brain Research, Vol. 731, No. 1-2, 26.08.1996, p. 39-44.

Research output: Contribution to journalArticle

Kane, Cynthia J.M. ; Berry, April ; Boop, Frederick ; Davies, David L. / Proliferation of astroglia from the adult human cerebrum is inhibited by ethanol in vitro. In: Brain Research. 1996 ; Vol. 731, No. 1-2. pp. 39-44.
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