Prostacyclin production by perturbed bovine aortic endothelial cells in culture

P. P. Nawroth, David Stern, K. L. Kaplan, H. L. Nossel

Research output: Contribution to journalArticle

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Abstract

This study reports that endotoxin (Escherichia coli serotype O26:B6) and 12-O-tetradecanoyl-phorbol-13-acetate stimulate cultured bovine aortic endothelial cells to generate prostacyclin. The prostacyclin concentration of the culture medium was measured indirectly by radioimmunoassay for 6-keto-PGF(1α). The amount of prostacyclin generated depended on the concentration of endotoxin or phorbol diester. Prostacyclin generation was not immediate, but occurred slowly after a six-hour lag period. The perturbed cells contracted and showed marked shape changes that correlated temporally with the start of enhanced prostacyclin production. Cytochalasins B and D, vinblastine, and colchicine inhibited prostacyclin production, indicating involvement of the cytoskeleton in the cellular response to endotoxin and phorbol diester. The increase in prostacyclin production was prevented by trifluoperazine, an inhibitor of the Ca++-calmodulin system, which is known to be involved in cytoskeletal function. Generation of prostacyclin was inhibited by cycloheximide and actinomycin D, indicating dependence on protein and ribonucleic acid synthesis. It is postulated that exposure to endotoxin or phorbol diester leads, via a series of reactions that involve RNA and protein synthesis and require intact cytoskeletal function, to the generation of toxic active intermediate(s) that stimulate the enzymes necessary for prostacyclin production.

Original languageEnglish (US)
Pages (from-to)801-806
Number of pages6
JournalBlood
Volume64
Issue number4
StatePublished - 1984
Externally publishedYes

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Endothelial cells
Epoprostenol
Cell culture
Endothelial Cells
Cell Culture Techniques
Phorbol Esters
Endotoxins
RNA
Cytochalasin D
Trifluoperazine
Cytochalasin B
Vinblastine
Poisons
Colchicine
Prostaglandins F
Dactinomycin
Tetradecanoylphorbol Acetate
Calmodulin
Cycloheximide
Cytoskeleton

All Science Journal Classification (ASJC) codes

  • Hematology

Cite this

Nawroth, P. P., Stern, D., Kaplan, K. L., & Nossel, H. L. (1984). Prostacyclin production by perturbed bovine aortic endothelial cells in culture. Blood, 64(4), 801-806.

Prostacyclin production by perturbed bovine aortic endothelial cells in culture. / Nawroth, P. P.; Stern, David; Kaplan, K. L.; Nossel, H. L.

In: Blood, Vol. 64, No. 4, 1984, p. 801-806.

Research output: Contribution to journalArticle

Nawroth, PP, Stern, D, Kaplan, KL & Nossel, HL 1984, 'Prostacyclin production by perturbed bovine aortic endothelial cells in culture', Blood, vol. 64, no. 4, pp. 801-806.
Nawroth, P. P. ; Stern, David ; Kaplan, K. L. ; Nossel, H. L. / Prostacyclin production by perturbed bovine aortic endothelial cells in culture. In: Blood. 1984 ; Vol. 64, No. 4. pp. 801-806.
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