Prostaglandin E2 regulates wound closure in airway epithelium

Ushma Savla, Heidi J. Appel, Peter H.S. Sporn, Christopher Waters

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Repair of the airway epithelium after injury is critical for the maintenance of barrier function and the limitation of airway hyperreactivity. Airway epithelial cells (AECs) metabolize arachidonic acid to biologically active eicosanoids via the enzyme cyclooxygenase (COX). We investigated whether stimulating or inhibiting COX metabolites would affect wound closure in monolayers of cultured AECs. Inhibiting COX with indomethacin resulted in a dose-dependent inhibition of wound closure in human and feline AECs. Specific inhibitors for both COX-1 and COX-2 isoforms impaired wound healing. Inhibitors of 5-lipoxygenase did not affect wound closure in these cells. The addition of prostaglandin E2 (PGE2) eliminated the inhibition due to indomethacin treatment, and the exogenous application of PGE2 stimulated wound closure in a dose-dependent manner. Inhibition of COX with indomethacin only at initial time points resulted in a sustained inhibition of wound closure, indicating that prostanoids are involved in early wound repair processes such as spreading and migration. These differences in wound closure may be important if arachidonic acid metabolism and eicosanoid concentrations are altered in disease states such as asthma.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume280
Issue number3 24-3
StatePublished - Mar 1 2001

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Dinoprostone
Epithelium
Wounds and Injuries
Prostaglandin-Endoperoxide Synthases
Indomethacin
Eicosanoids
Epithelial Cells
Arachidonic Acid
Lipoxygenase Inhibitors
Cyclooxygenase 1
Felidae
Cyclooxygenase 2
Wound Healing
Prostaglandins
Protein Isoforms
Asthma
Maintenance
Enzymes

All Science Journal Classification (ASJC) codes

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

Cite this

Prostaglandin E2 regulates wound closure in airway epithelium. / Savla, Ushma; Appel, Heidi J.; Sporn, Peter H.S.; Waters, Christopher.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 280, No. 3 24-3, 01.03.2001.

Research output: Contribution to journalArticle

Savla, Ushma ; Appel, Heidi J. ; Sporn, Peter H.S. ; Waters, Christopher. / Prostaglandin E2 regulates wound closure in airway epithelium. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 2001 ; Vol. 280, No. 3 24-3.
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