Prostaglandin production after experimental discectomy

James T. Robertson, George V. Huffmon, L. Brannon Thomas, Charles Leffler, Brett C. Gunter, Richard P. White

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Study Design. This study ascertained the effects of discectomy on prostaglandin synthesis. Objectives. The purpose of these novel experiments was to measure the levels of two prostaglandins in lumbar epidural fluid obtained from an area subjected to discectomy. For comparison, lumbar epidural fluid from a site not disturbed by discectomy and fluid from a subcutaneous site were analyzed for the prostaglandins. Summary of Background Data. Previous studies have shown that nuclear material obtained from degenerative discs manifests an extraordinarily high level of phospholipase A2 activity. Others have hypothesized that the known inflammatory effects of phospholipase A2 are due to the release of arachidonic acid, which is converted to various eicosanoids, including several algesic prostaglandins (PGI2 and PGE2). No previous study has continuously measured prostaglandin levels in epidural fluid or assessed the effect of discectomy on prostaglandin production. Methods. An ultrafiltrate of lumbar epidural fluid of dogs was obtained from indwelling catheters located adjacent to spinal areas that were and were not subjected to discectomy as well as from subcutaneous tissue. The fluid was collected daily for 14 days and analyzed for PGE2 and 6-keto PGF(1α) (the stable metabolite of PGI2) by radioimmunoassay. Results. The concentration of 6-keto PGF(1α) and PGE2 in fluid collected during the first 24 hours was significantly higher in the area of discectomy than in the epidural region that was not subjected to discectomy and significantly higher than in fluid obtained from the subcutaneous site. The high level of these prostaglandins at the discectomy site fell rapidly, so that by the end of 48 hours the differences in values between spinal fluid from the discectomy and nondiscectomy regions were not statistically significant. The concentration of the prostaglandins in epidural fluid decreased with time and became minimal within the second week. Conclusion. The removal of normal discs is accompanied for 24 hours by a marked rise in the synthesis of two prostaglandins known to produce pain. Because the concentration of prostaglandins in epidural fluid decreased rapidly thereafter, the initial surge obtained appears to be associated more with chemical factors such as phospholipase A2 than with wound healing.

Original languageEnglish (US)
Pages (from-to)1731-1736
Number of pages6
JournalSpine
Volume21
Issue number15
DOIs
StatePublished - Aug 1 1996

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Diskectomy
Prostaglandins
Phospholipases A2
Dinoprostone
Prostaglandins F
Epoprostenol
Indwelling Catheters
Eicosanoids
Subcutaneous Tissue
Arachidonic Acid
Wound Healing
Radioimmunoassay
Dogs
Pain

All Science Journal Classification (ASJC) codes

  • Orthopedics and Sports Medicine
  • Clinical Neurology

Cite this

Robertson, J. T., Huffmon, G. V., Thomas, L. B., Leffler, C., Gunter, B. C., & White, R. P. (1996). Prostaglandin production after experimental discectomy. Spine, 21(15), 1731-1736. https://doi.org/10.1097/00007632-199608010-00004

Prostaglandin production after experimental discectomy. / Robertson, James T.; Huffmon, George V.; Thomas, L. Brannon; Leffler, Charles; Gunter, Brett C.; White, Richard P.

In: Spine, Vol. 21, No. 15, 01.08.1996, p. 1731-1736.

Research output: Contribution to journalArticle

Robertson, JT, Huffmon, GV, Thomas, LB, Leffler, C, Gunter, BC & White, RP 1996, 'Prostaglandin production after experimental discectomy', Spine, vol. 21, no. 15, pp. 1731-1736. https://doi.org/10.1097/00007632-199608010-00004
Robertson JT, Huffmon GV, Thomas LB, Leffler C, Gunter BC, White RP. Prostaglandin production after experimental discectomy. Spine. 1996 Aug 1;21(15):1731-1736. https://doi.org/10.1097/00007632-199608010-00004
Robertson, James T. ; Huffmon, George V. ; Thomas, L. Brannon ; Leffler, Charles ; Gunter, Brett C. ; White, Richard P. / Prostaglandin production after experimental discectomy. In: Spine. 1996 ; Vol. 21, No. 15. pp. 1731-1736.
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