Prostanoid synthesis and vascular responses to exogenous arachidonic acid following cerebral ischemia in piglets

Charles Leffler, R. Mirro, W. M. Armstead, D. W. Busija, O. Thelin

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

In newborn pigs, cerebral ischemia abolishes both increased cerebral prostanoid production and cerebral vasodilation in response to hypercapnia and hypotension. Attenuation of prostaglandin endoperoxide synthase activity could account for the failure to increase prostanoid systhesis and loss of responses to these stimuli. To test this possibility, arachidonic acid (3,6, or 30μg/ml) was placed under cranial windows in newborn pigs that been exposed to 20 min of cerebral ischemia. The conversion to prostanoids and pial arteriolar responses to the arachidonic acid were measured. At all three concentration, arachidonic acid caused similar increases in pial arteriolar diameter in sham control piglets and piglets 1 hr postischemia. Topical arachidonic acid caused dosedependent increases of PGE2 in cortical periarachnoid cerebral spinal fluid. 6-keto-PGF and TXB2 only increased at the highest concentration of arachidonic acid (30 μg/ml). Cerebral ischemia did not decrease the conservation of any concentration of arachidonic acid to PGE2, 6-keto-PGF, or TXB2. We conclude that ischemia and subsequent reperfusion do not result in inhibition of prostaglandin endoperoxide synthase in the newborn pig brain. Therefore, the mechanism for the impaired prostanoid production in response to hypercapnia and hypotension following cerebral ischemia appears to involve reduction in release of free arachidonic acid.

Original languageEnglish (US)
Pages (from-to)241-248
Number of pages8
JournalProstaglandins
Volume40
Issue number3
DOIs
StatePublished - Jan 1 1990

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Brain Ischemia
Arachidonic Acid
Prostaglandins
Blood Vessels
Swine
Hypercapnia
Prostaglandin-Endoperoxide Synthases
Dinoprostone
Hypotension
Vasodilation
Reperfusion
Conservation
Brain
Ischemia
Fluids

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Endocrinology

Cite this

Prostanoid synthesis and vascular responses to exogenous arachidonic acid following cerebral ischemia in piglets. / Leffler, Charles; Mirro, R.; Armstead, W. M.; Busija, D. W.; Thelin, O.

In: Prostaglandins, Vol. 40, No. 3, 01.01.1990, p. 241-248.

Research output: Contribution to journalArticle

Leffler, Charles ; Mirro, R. ; Armstead, W. M. ; Busija, D. W. ; Thelin, O. / Prostanoid synthesis and vascular responses to exogenous arachidonic acid following cerebral ischemia in piglets. In: Prostaglandins. 1990 ; Vol. 40, No. 3. pp. 241-248.
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