Protection of calcitonin gene-related peptide-mediated preconditioning against coronary endothelial dysfunction induced by reperfusion in the isolated rat heart

Fuwen Zhou, Yuan Jian Li, Rong Lu, Han Wu Deng

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

This study was designed to explore the protective effect of ischemic preconditioning on reperfusion-induced coronary endothelial dysfunction, with a focus on the role of calcitonin gene-related peptide (CGRP) in this effect, in the isolated perfused rat heart. Thirty minutes of global ischemia and 30 min of reperfusion significantly decreased heart rate, left ventricular pressure, and its first derivative and impaired vasodilator responses to acetylcholine. Ischemia-reperfusion did not affect vasodilator responses to sodium nitroprusside. Preconditioning induced by three cycles of 5 min of ischemia and 5 min of reperfusion produced a significant improvement in cardiac function concomitantly with an amelioration of vasodilator responses to acetylcholine. The protective effects of ischemic preconditioning were abolished by CGRP8-37 (10-7 M), the selective CGRP receptor antagonist. After pretreatment with capsaicin (50 mg · kg-1, s.c.) to deplete endogenous CGRP, the preconditioning effect was absent. Pretreatment with exogenous CGRP (5 X 10-9 M) for 5 min induced a preconditioning-like protection. The present study suggests that the cardioprotection of ischemic preconditioning is related to the preservation of the coronary endothelial cell, and that the protective effect of preconditioning is mediated by endogenous CGRP in the isolated perfused rat heart.

Original languageEnglish (US)
Pages (from-to)1091-1097
Number of pages7
JournalLife Sciences
Volume64
Issue number13
DOIs
StatePublished - Feb 19 1999

Fingerprint

Calcitonin Gene-Related Peptide
Ischemic Preconditioning
Reperfusion
Rats
Vasodilator Agents
Ischemia
Acetylcholine
Calcitonin Gene-Related Peptide Receptors
Myocardial Reperfusion
Capsaicin
Endothelial cells
Nitroprusside
Ventricular Pressure
Endothelial Cells
Heart Rate
Derivatives

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Protection of calcitonin gene-related peptide-mediated preconditioning against coronary endothelial dysfunction induced by reperfusion in the isolated rat heart. / Zhou, Fuwen; Li, Yuan Jian; Lu, Rong; Deng, Han Wu.

In: Life Sciences, Vol. 64, No. 13, 19.02.1999, p. 1091-1097.

Research output: Contribution to journalArticle

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