Protective immunity evoked by locally administered group A streptococcal vaccines in mice

M. S. Bronze, D. S. McKinsey, E. H. Beachey, James Dale

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The present studies were undertaken to determine the pathogenicity of group A streptococci introduced intranasally (i.n.) into mice in an attempt to mimic mucosal infections in humans and to determine the efficay of streptococcal vaccines administered via the mucosal route. The LD50 of type 24 streptococci (M24 strep) administered i.n. was 3 x 104 CFU. Throat cultures were performed in M24 strep-inoculated mice. Of 11 mice that died, 9 had positive throat cultures 3 or 4 days after i.n. challenge, and of 9 mice that survived, only 1 had a positive throat culture, indicating an association between mucosal infection and death. Postmortem examination performed on 35 mice that died after i.n. challenge showed that all had evidence of disseminated infections, and group A streptococci were recovered from the cervical lymph nodes, blood, spleen, liver, and brain. To determine vaccine efficacy, heat-killed M24 strep or pep M24 were administered iln. to groups of mice. While, heat-killed streptococci and pep M24 administered locally protected mice against death from i.n. challenge infections with homologous M24 strep. The whole cell vaccine also protected against i.n. challenge infections with heterologous type 6 streptococci. Our data suggest that streptococcal vaccines administered locally evoke protective immunity against streptococcal infections.

Original languageEnglish (US)
Pages (from-to)2767-2770
Number of pages4
JournalJournal of Immunology
Volume141
Issue number8
StatePublished - Jan 1 1988
Externally publishedYes

Fingerprint

Streptococcal Vaccines
Immunity
Streptococcus
Pharynx
Infection
Vaccines
Hot Temperature
Streptococcal Infections
Lethal Dose 50
Virulence
Autopsy
Spleen
Lymph Nodes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Protective immunity evoked by locally administered group A streptococcal vaccines in mice. / Bronze, M. S.; McKinsey, D. S.; Beachey, E. H.; Dale, James.

In: Journal of Immunology, Vol. 141, No. 8, 01.01.1988, p. 2767-2770.

Research output: Contribution to journalArticle

Bronze, MS, McKinsey, DS, Beachey, EH & Dale, J 1988, 'Protective immunity evoked by locally administered group A streptococcal vaccines in mice', Journal of Immunology, vol. 141, no. 8, pp. 2767-2770.
Bronze, M. S. ; McKinsey, D. S. ; Beachey, E. H. ; Dale, James. / Protective immunity evoked by locally administered group A streptococcal vaccines in mice. In: Journal of Immunology. 1988 ; Vol. 141, No. 8. pp. 2767-2770.
@article{821b04844e8a4b029abefef02ae02399,
title = "Protective immunity evoked by locally administered group A streptococcal vaccines in mice",
abstract = "The present studies were undertaken to determine the pathogenicity of group A streptococci introduced intranasally (i.n.) into mice in an attempt to mimic mucosal infections in humans and to determine the efficay of streptococcal vaccines administered via the mucosal route. The LD50 of type 24 streptococci (M24 strep) administered i.n. was 3 x 104 CFU. Throat cultures were performed in M24 strep-inoculated mice. Of 11 mice that died, 9 had positive throat cultures 3 or 4 days after i.n. challenge, and of 9 mice that survived, only 1 had a positive throat culture, indicating an association between mucosal infection and death. Postmortem examination performed on 35 mice that died after i.n. challenge showed that all had evidence of disseminated infections, and group A streptococci were recovered from the cervical lymph nodes, blood, spleen, liver, and brain. To determine vaccine efficacy, heat-killed M24 strep or pep M24 were administered iln. to groups of mice. While, heat-killed streptococci and pep M24 administered locally protected mice against death from i.n. challenge infections with homologous M24 strep. The whole cell vaccine also protected against i.n. challenge infections with heterologous type 6 streptococci. Our data suggest that streptococcal vaccines administered locally evoke protective immunity against streptococcal infections.",
author = "Bronze, {M. S.} and McKinsey, {D. S.} and Beachey, {E. H.} and James Dale",
year = "1988",
month = "1",
day = "1",
language = "English (US)",
volume = "141",
pages = "2767--2770",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "8",

}

TY - JOUR

T1 - Protective immunity evoked by locally administered group A streptococcal vaccines in mice

AU - Bronze, M. S.

AU - McKinsey, D. S.

AU - Beachey, E. H.

AU - Dale, James

PY - 1988/1/1

Y1 - 1988/1/1

N2 - The present studies were undertaken to determine the pathogenicity of group A streptococci introduced intranasally (i.n.) into mice in an attempt to mimic mucosal infections in humans and to determine the efficay of streptococcal vaccines administered via the mucosal route. The LD50 of type 24 streptococci (M24 strep) administered i.n. was 3 x 104 CFU. Throat cultures were performed in M24 strep-inoculated mice. Of 11 mice that died, 9 had positive throat cultures 3 or 4 days after i.n. challenge, and of 9 mice that survived, only 1 had a positive throat culture, indicating an association between mucosal infection and death. Postmortem examination performed on 35 mice that died after i.n. challenge showed that all had evidence of disseminated infections, and group A streptococci were recovered from the cervical lymph nodes, blood, spleen, liver, and brain. To determine vaccine efficacy, heat-killed M24 strep or pep M24 were administered iln. to groups of mice. While, heat-killed streptococci and pep M24 administered locally protected mice against death from i.n. challenge infections with homologous M24 strep. The whole cell vaccine also protected against i.n. challenge infections with heterologous type 6 streptococci. Our data suggest that streptococcal vaccines administered locally evoke protective immunity against streptococcal infections.

AB - The present studies were undertaken to determine the pathogenicity of group A streptococci introduced intranasally (i.n.) into mice in an attempt to mimic mucosal infections in humans and to determine the efficay of streptococcal vaccines administered via the mucosal route. The LD50 of type 24 streptococci (M24 strep) administered i.n. was 3 x 104 CFU. Throat cultures were performed in M24 strep-inoculated mice. Of 11 mice that died, 9 had positive throat cultures 3 or 4 days after i.n. challenge, and of 9 mice that survived, only 1 had a positive throat culture, indicating an association between mucosal infection and death. Postmortem examination performed on 35 mice that died after i.n. challenge showed that all had evidence of disseminated infections, and group A streptococci were recovered from the cervical lymph nodes, blood, spleen, liver, and brain. To determine vaccine efficacy, heat-killed M24 strep or pep M24 were administered iln. to groups of mice. While, heat-killed streptococci and pep M24 administered locally protected mice against death from i.n. challenge infections with homologous M24 strep. The whole cell vaccine also protected against i.n. challenge infections with heterologous type 6 streptococci. Our data suggest that streptococcal vaccines administered locally evoke protective immunity against streptococcal infections.

UR - http://www.scopus.com/inward/record.url?scp=0023812251&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023812251&partnerID=8YFLogxK

M3 - Article

VL - 141

SP - 2767

EP - 2770

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 8

ER -