Protein kinase A in the pedunculopontine tegmental nucleus of rat contributes to regulation of rapid eye movement sleep

Subimal Datta, Frank Desarnaud

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Intracellular signaling mechanisms within the pedunculopontine tegmental (PPT) nucleus for the regulation of recovery rapid eye movement (REM) sleep following REM sleep deprivation remain unknown. This study was designed to determine the role of PPT intracellular cAMP-dependent protein kinase A (cAMP-PKA) in the regulation of recovery REM sleep in freely moving rats. The results show that a brief period (3 h) of selective REM sleep deprivation caused REM sleep rebound associated with increased PKA activity and expression of the PKA catalytic subunit protein (PKA-CU) in the PPT. Local application of a cAMP-PKA-activation-selective inhibitor, RpCAMPS (0.55, 1.1, and 2.2 nmol/100 nl; n = 8 rats/group), bilaterally into the PPT, reduced PKA activity and PKA-CU expression in the PPT, and suppressed the recovery REM sleep, in a dose-dependent manner. Regression analyses revealed significant positive relationships between: PPT levels of PKA activity and the total percentages of REM sleep recovery (Rsqr = 0.944; n = 40 rats); PPT levels of PKA-CU expression and the total percentages of REM sleep recovery (Rsqr = 0.937; n = 40 rats); PPT levels of PKA-CU expression and PKA activity (Rsqr = 0.945; n = 40 rats). Collectively, these results provide evidence that activation of intracellular PKA in the PPT contributes to REM sleep recovery following REM sleep deprivation.

Original languageEnglish (US)
Pages (from-to)12263-12273
Number of pages11
JournalJournal of Neuroscience
Volume30
Issue number37
DOIs
StatePublished - Sep 15 2010

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Pedunculopontine Tegmental Nucleus
REM Sleep
Cyclic AMP-Dependent Protein Kinases
Sleep
Sleep Deprivation
Catalytic Domain

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Protein kinase A in the pedunculopontine tegmental nucleus of rat contributes to regulation of rapid eye movement sleep. / Datta, Subimal; Desarnaud, Frank.

In: Journal of Neuroscience, Vol. 30, No. 37, 15.09.2010, p. 12263-12273.

Research output: Contribution to journalArticle

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