Protein kinase Cζ regulates phospholipase D activity in rat-1 fibroblasts expressing the α 1A adrenergic receptor

Jean Hugues Parmentier, Gautam K. Gandhi, Monique T. Wiggins, Abdelwahab E. Saeed, Sylvain G. Bourgoin, Kafait Malik

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: Phenylephrine (PHE), an α 1 adrenergic receptor agonist, increases phospholipase D (PLD) activity, independent of classical and novel protein kinase C (PKC) isoforms, in rat-1 fibroblasts expressing α 1A adrenergic receptors. The aim of this study was to determine the contribution of atypical PKCζ to PLD activation in response to PHE in these cells. Results: PHE stimulated a PLD activity as demonstrated by phosphatidylethanol production. PHE increased PKCζ translocation to the particulate cell fraction in parallel with a time-dependent decrease in its activity. PKCζ activity was reduced at 2 and 5 min and returned to a sub-basal level within 10-15 min. Ectopic expression of kinase-dead PKCζ, but not constitutively active PKCζ, potentiated PLD activation elicited by PHE. A cell-permeable pseudosubstrate inhibitor of PKCζ reduced basal PKCζ activity and abolished PHE-induced PLD activation. Conclusion: α 1A adrenergic receptor stimulation promotes the activation of a PLD activity by a mechanism dependent on PKCζ; Our data also suggest that catalytic activation of PKCζ is not required for PLD stimulation.

Original languageEnglish (US)
Article number4
JournalBMC cell biology
Volume5
DOIs
StatePublished - Jan 8 2004

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Phospholipase D
Adrenergic Receptors
Protein Kinase C
Phenylephrine
Fibroblasts
Adrenergic Agonists
Protein Isoforms

All Science Journal Classification (ASJC) codes

  • Cell Biology

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Protein kinase Cζ regulates phospholipase D activity in rat-1 fibroblasts expressing the α 1A adrenergic receptor . / Parmentier, Jean Hugues; Gandhi, Gautam K.; Wiggins, Monique T.; Saeed, Abdelwahab E.; Bourgoin, Sylvain G.; Malik, Kafait.

In: BMC cell biology, Vol. 5, 4, 08.01.2004.

Research output: Contribution to journalArticle

Parmentier, Jean Hugues ; Gandhi, Gautam K. ; Wiggins, Monique T. ; Saeed, Abdelwahab E. ; Bourgoin, Sylvain G. ; Malik, Kafait. / Protein kinase Cζ regulates phospholipase D activity in rat-1 fibroblasts expressing the α 1A adrenergic receptor In: BMC cell biology. 2004 ; Vol. 5.
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AU - Bourgoin, Sylvain G.

AU - Malik, Kafait

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