Protein kinase D1

A new component in TLR9 signaling

Jeoung Eun Park, Young In Kim Hoehamer, Ae-Kyung Yi

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Protein kinase D1 (PKD1) is expressed ubiquitously and regulates diverse cellular processes such as oxidative stress, gene expression, cell survival, and vesicle trafficking. However, the presence and function of PKD1 in monocytic cells are currently unknown. In this study, we provide evidence that PKD1 is involved in TLR9 signaling in macrophages. Class B-type CpG DNA (CpG-B DNA) induced activation of PKD1 via a pathway that is dependent on endosomal pH, TLR9, MyD88, and IL-1R-associated kinase 1 in macrophages. Upon CpG-B DNA stimulation, PKD1 interacted with the TLR9/MyD88/IL-1R-associated kinase/TNFR-associated factor 6 complex. Knockdown of PKD1 revealed that PKD1 is required for activation of NF-κB and MAPKs, and subsequent expression of cytokines in response to CpG-B DNA. Our findings identify PKD1 as a key signaling modulator in TLR9-mediated macrophage activation.

Original languageEnglish (US)
Pages (from-to)2044-2055
Number of pages12
JournalJournal of Immunology
Volume181
Issue number3
DOIs
StatePublished - Aug 1 2008

Fingerprint

Protein Kinases
DNA
Phosphotransferases
Macrophages
Macrophage Activation
Cell Survival
Oxidative Stress
Cytokines
Gene Expression

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Protein kinase D1 : A new component in TLR9 signaling. / Park, Jeoung Eun; Kim Hoehamer, Young In; Yi, Ae-Kyung.

In: Journal of Immunology, Vol. 181, No. 3, 01.08.2008, p. 2044-2055.

Research output: Contribution to journalArticle

Park, Jeoung Eun ; Kim Hoehamer, Young In ; Yi, Ae-Kyung. / Protein kinase D1 : A new component in TLR9 signaling. In: Journal of Immunology. 2008 ; Vol. 181, No. 3. pp. 2044-2055.
@article{f9d76788d31e49bd9302fc2da6984d57,
title = "Protein kinase D1: A new component in TLR9 signaling",
abstract = "Protein kinase D1 (PKD1) is expressed ubiquitously and regulates diverse cellular processes such as oxidative stress, gene expression, cell survival, and vesicle trafficking. However, the presence and function of PKD1 in monocytic cells are currently unknown. In this study, we provide evidence that PKD1 is involved in TLR9 signaling in macrophages. Class B-type CpG DNA (CpG-B DNA) induced activation of PKD1 via a pathway that is dependent on endosomal pH, TLR9, MyD88, and IL-1R-associated kinase 1 in macrophages. Upon CpG-B DNA stimulation, PKD1 interacted with the TLR9/MyD88/IL-1R-associated kinase/TNFR-associated factor 6 complex. Knockdown of PKD1 revealed that PKD1 is required for activation of NF-κB and MAPKs, and subsequent expression of cytokines in response to CpG-B DNA. Our findings identify PKD1 as a key signaling modulator in TLR9-mediated macrophage activation.",
author = "Park, {Jeoung Eun} and {Kim Hoehamer}, {Young In} and Ae-Kyung Yi",
year = "2008",
month = "8",
day = "1",
doi = "10.4049/jimmunol.181.3.2044",
language = "English (US)",
volume = "181",
pages = "2044--2055",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "3",

}

TY - JOUR

T1 - Protein kinase D1

T2 - A new component in TLR9 signaling

AU - Park, Jeoung Eun

AU - Kim Hoehamer, Young In

AU - Yi, Ae-Kyung

PY - 2008/8/1

Y1 - 2008/8/1

N2 - Protein kinase D1 (PKD1) is expressed ubiquitously and regulates diverse cellular processes such as oxidative stress, gene expression, cell survival, and vesicle trafficking. However, the presence and function of PKD1 in monocytic cells are currently unknown. In this study, we provide evidence that PKD1 is involved in TLR9 signaling in macrophages. Class B-type CpG DNA (CpG-B DNA) induced activation of PKD1 via a pathway that is dependent on endosomal pH, TLR9, MyD88, and IL-1R-associated kinase 1 in macrophages. Upon CpG-B DNA stimulation, PKD1 interacted with the TLR9/MyD88/IL-1R-associated kinase/TNFR-associated factor 6 complex. Knockdown of PKD1 revealed that PKD1 is required for activation of NF-κB and MAPKs, and subsequent expression of cytokines in response to CpG-B DNA. Our findings identify PKD1 as a key signaling modulator in TLR9-mediated macrophage activation.

AB - Protein kinase D1 (PKD1) is expressed ubiquitously and regulates diverse cellular processes such as oxidative stress, gene expression, cell survival, and vesicle trafficking. However, the presence and function of PKD1 in monocytic cells are currently unknown. In this study, we provide evidence that PKD1 is involved in TLR9 signaling in macrophages. Class B-type CpG DNA (CpG-B DNA) induced activation of PKD1 via a pathway that is dependent on endosomal pH, TLR9, MyD88, and IL-1R-associated kinase 1 in macrophages. Upon CpG-B DNA stimulation, PKD1 interacted with the TLR9/MyD88/IL-1R-associated kinase/TNFR-associated factor 6 complex. Knockdown of PKD1 revealed that PKD1 is required for activation of NF-κB and MAPKs, and subsequent expression of cytokines in response to CpG-B DNA. Our findings identify PKD1 as a key signaling modulator in TLR9-mediated macrophage activation.

UR - http://www.scopus.com/inward/record.url?scp=49649096410&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=49649096410&partnerID=8YFLogxK

U2 - 10.4049/jimmunol.181.3.2044

DO - 10.4049/jimmunol.181.3.2044

M3 - Article

VL - 181

SP - 2044

EP - 2055

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 3

ER -