Protein kinase D1 (PKD1) influences androgen receptor (AR) function in prostate cancer cells

Paul Mak, Meena Jaggi, Viqar Syed, Subhash Chauhan, Sazzad Hassan, Helal Biswas, K. C. Balaji

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Protein kinase D1 (PKD1), founding member of PKD protein family, is down-regulated in advanced prostate cancer (PCa). We demonstrate that PKD1 and androgen receptor (AR) are present as a protein complex in PCa cells. PKD1 is associated with a transcriptional complex which contains AR and promoter sequence of the Prostate Specific Antigen (PSA) gene. Ectopic expression of wild type PKD1 and the kinase dead mutant PKD1 (K628W) attenuated the ligand-dependent transcriptional activation of AR in prostate cancer cells and yeast cells indicating that PKD1 can affect AR transcription activity, whereas knocking down PKD1 enhanced the ligand-dependent transcriptional activation of AR. Co-expression of kinase dead mutant with AR significantly inhibited androgen-mediated cell proliferation in both LNCaP and DU145 PC cells. Our data demonstrate for the first time that PKD1 can influence AR function in PCa cells.

Original languageEnglish (US)
Pages (from-to)618-623
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume373
Issue number4
DOIs
StatePublished - Sep 5 2008
Externally publishedYes

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Androgen Receptors
Protein Kinases
Prostatic Neoplasms
Cells
Transcriptional Activation
Phosphotransferases
Chemical activation
Ligands
Cell proliferation
Mutant Proteins
Transcription
Prostate-Specific Antigen
Yeast
Androgens
Proteins
Genes
Yeasts
Cell Proliferation

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Protein kinase D1 (PKD1) influences androgen receptor (AR) function in prostate cancer cells. / Mak, Paul; Jaggi, Meena; Syed, Viqar; Chauhan, Subhash; Hassan, Sazzad; Biswas, Helal; Balaji, K. C.

In: Biochemical and Biophysical Research Communications, Vol. 373, No. 4, 05.09.2008, p. 618-623.

Research output: Contribution to journalArticle

Mak, Paul ; Jaggi, Meena ; Syed, Viqar ; Chauhan, Subhash ; Hassan, Sazzad ; Biswas, Helal ; Balaji, K. C. / Protein kinase D1 (PKD1) influences androgen receptor (AR) function in prostate cancer cells. In: Biochemical and Biophysical Research Communications. 2008 ; Vol. 373, No. 4. pp. 618-623.
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