Protein-lipid interactions and enzyme requirements for light subtype generation on cycling reconstituted surfactant

Rajiv Dhand, Vijay K. Sharma, Andelle L. Teng, S. Krishnasamy, Nicholas J. Gross

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Surfactant convertase is required for conversion of heavy density (H) natural surfactant to light density (L) subtype during cycling in vitro, a technique that reproduces surfactant metabolism. To study mechanisms of H to L conversion, we prepared liposomes of dipalmitoylphosphatidylcholine (DPPC) and phosphatidylglycerol (PG), or the phospholipids (PL) in combination with either surfactant protein A (SP-A), surfactant protein B (SP-B), or both SP-A and SP-B. Phospholipids alone showed time-dependent conversion from heavy to light subtype on cycling in the absence of convertase, which was decreased by adding SP-B, but not SP-A, to phospholipids (p < 0.01 for PL + SP-B, or PL + SP-A + SP-B vs. PL, or PL + SP-A). The ultrastructure, surface activity, buoyant density, and L subtype generation on cycling PL + SP-A + SP-B with partially purified convertase or with phospholipase D were similar to those of natural TM. In conclusion, a reconstituted surfactant mimics the behavior of natural surfactant on cycling, and reveals that interaction of SP-B with phospholipids decreases L subtype generation. In addition, esterase/phospholipase D activity is required for conversion of heavy to light subtype on cycling.

Original languageEnglish (US)
Pages (from-to)712-719
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume244
Issue number3
DOIs
StatePublished - Mar 27 1998
Externally publishedYes

Fingerprint

Surface-Active Agents
Pulmonary Surfactant-Associated Protein A
Phospholipids
Lipids
Light
Enzymes
Proteins
Phospholipase D
Pulmonary Surfactant-Associated Protein B
1,2-Dipalmitoylphosphatidylcholine
Phosphatidylglycerols
Esterases
Metabolism
Liposomes
IgA receptor

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Protein-lipid interactions and enzyme requirements for light subtype generation on cycling reconstituted surfactant. / Dhand, Rajiv; Sharma, Vijay K.; Teng, Andelle L.; Krishnasamy, S.; Gross, Nicholas J.

In: Biochemical and Biophysical Research Communications, Vol. 244, No. 3, 27.03.1998, p. 712-719.

Research output: Contribution to journalArticle

Dhand, Rajiv ; Sharma, Vijay K. ; Teng, Andelle L. ; Krishnasamy, S. ; Gross, Nicholas J. / Protein-lipid interactions and enzyme requirements for light subtype generation on cycling reconstituted surfactant. In: Biochemical and Biophysical Research Communications. 1998 ; Vol. 244, No. 3. pp. 712-719.
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