Protein Profiling in Brain Tumors Using Mass Spectrometry

Feasibility of a New Technique for the Analysis of Protein Expression

Sarah A. Schwartz, Robert J. Weil, Mahlon Johnson, Steven A. Toms, Richard M. Caprioli

Research output: Contribution to journalArticle

171 Citations (Scopus)

Abstract

Purpose: The purpose of this research was to perform a preliminary assessment of protein patterns in primary brain tumors using a direct-tissue mass spectrometric technique to profile and map biomolecules. Experimental Design: We examined 20 prospectively collected, snap-frozen normal brain and brain tumor specimens using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS), and compared peptide and protein expression in primary brain tumor and nontumor brain tissues. Results: MS can be used to identify protein expression patterns in human brain tissue and tumor specimens. The mass spectral patterns can reliably identify glial neoplasms of similar histological grade and differentiate them from tumors of different histological grades as well as from nontumor brain tissues. Initial bioinformatics cluster analysis algorithms classified tumor and nontumor tissues into similar groups comparable with their histological grade. Conclusions: We describe a novel tool for the analysis of protein expression patterns in human glial neoplasms. Initial results demonstrate that MALDI-MS technology can significantly aid in the process of unraveling and understanding the molecular complexities of gliomas. MALDI-MS accurately and reliably identified normal and neoplastic tissues, and could be used to discriminate between tumors of increasing grades.

Original languageEnglish (US)
Pages (from-to)981-987
Number of pages7
JournalClinical Cancer Research
Volume10
Issue number3
DOIs
StatePublished - Feb 1 2004

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Brain Neoplasms
Mass Spectrometry
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Proteins
Neoplasms
Neuroglia
Brain
Computational Biology
Glioma
Cluster Analysis
Research Design
Technology
Peptides
Research

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Protein Profiling in Brain Tumors Using Mass Spectrometry : Feasibility of a New Technique for the Analysis of Protein Expression. / Schwartz, Sarah A.; Weil, Robert J.; Johnson, Mahlon; Toms, Steven A.; Caprioli, Richard M.

In: Clinical Cancer Research, Vol. 10, No. 3, 01.02.2004, p. 981-987.

Research output: Contribution to journalArticle

Schwartz, Sarah A. ; Weil, Robert J. ; Johnson, Mahlon ; Toms, Steven A. ; Caprioli, Richard M. / Protein Profiling in Brain Tumors Using Mass Spectrometry : Feasibility of a New Technique for the Analysis of Protein Expression. In: Clinical Cancer Research. 2004 ; Vol. 10, No. 3. pp. 981-987.
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