Protein profiling of sickle cell versus control rbc core membrane skeletons by icat technology and tandem mass spectrometry

Jose Chou, Pankaj K. Choudhary, Steven Goodman

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A proteomic approach using a cleavable ICAT reagent and nano-LC ESI tandem mass spectrometry was used to perform protein profiling of core RBC membrane skeleton proteins between sickle cell patients (SS) and controls (AA), and determine the efficacy of this technology. The data was validated through Peptide/Protein Prophet and protein ratios were calculated through ASAPratio. Through an ANOVA test, it was determined that there is no significant difference in the mean ratios from control populations (AA1/AA2) and sickle cell versus control populations (AA/SS). The mean ratios were not significantly different from 1.0 in either comparison for the core skeleton proteins (α spectrin, β spectrin, band 4.1 and actin). On the natural-log scale, the variation (standard deviation) of the method was determined to be 14.1% and the variation contributed by the samples was 13.8% which together give a total variation of 19.7% in the ratios.

Original languageEnglish (US)
Pages (from-to)326-337
Number of pages12
JournalCellular and Molecular Biology Letters
Volume11
Issue number3
DOIs
StatePublished - Jan 1 2006

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Tandem Mass Spectrometry
Skeleton
Mass spectrometry
Technology
Membranes
Spectrin
Proteins
Electrospray Ionization Mass Spectrometry
Proteomics
Population
Actins
Analysis of Variance
Analysis of variance (ANOVA)
Membrane Proteins
Peptides

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Protein profiling of sickle cell versus control rbc core membrane skeletons by icat technology and tandem mass spectrometry. / Chou, Jose; Choudhary, Pankaj K.; Goodman, Steven.

In: Cellular and Molecular Biology Letters, Vol. 11, No. 3, 01.01.2006, p. 326-337.

Research output: Contribution to journalArticle

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