Protein quality and the protein to carbohydrate ratio within a high fat diet influences energy balance and the gut microbiota in C57BL/6J mice

Liam Mcallan, Peter Skuse, Paul D. Cotter, Paula O'Connor, John F. Cryan, R. Paul Ross, Gerald Fitzgerald, Helen M. Roche, Kanishka N. Nilaweera

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Macronutrient quality and composition are important determinants of energy balance and the gut microbiota. Here, we investigated how changes to protein quality (casein versus whey protein isolate; WPI) and the protein to carbohydrate (P/C) ratio within a high fat diet (HFD) impacts on these parameters. Mice were fed a low fat diet (10% kJ) or a high fat diet (HFD; 45% kJ) for 21 weeks with either casein (20% kJ, HFD) or WPI at 20%, 30% or 40% kJ. In comparison to casein, WPI at a similar energy content normalised energy intake, increased lean mass and caused a trend towards a reduction in fat mass (P = 0.08), but the protein challenge did not alter oxygen consumption or locomotor activity. WPI reduced HFD-induced plasma leptin and liver triacylglycerol, and partially attenuated the reduction in adipose FASN mRNA in HFD-fed mice. High throughput sequence-based analysis of faecal microbial populations revealed microbiota in the HFD-20% WPI group clustering closely with HFD controls, although WPI specifically increased Lactobacillaceae/ Lactobacillus and decreased Clostridiaceae/Clostridium in HFD-fed mice. There was no effect of increasing the P/C ratio on energy intake, but the highest ratio reduced HFD-induced weight gain, fat mass and plasma triacylglycerol, non-esterified fatty acids, glucose and leptin levels, while it increased lean mass and oxygen consumption. Similar effects were observed on adipose mRNA expression, where the highest ratio reduced HFD-associated expression of UCP-2, TNFα and CD68 and increased the diet-associated expression of β3-AR, LPL, IR, IRS-1 and GLUT4. The P/C ratio also impacted on gut microbiota, with populations in the 30/40% WPI groups clustering together and away from the 20% WPI group. Taken together, our data show that increasing the P/C ratio has a dramatic effect on energy balance and the composition of gut microbiota, which is distinct from that caused by changes to protein quality.

Original languageEnglish (US)
Article numbere88904
JournalPloS one
Volume9
Issue number2
DOIs
StatePublished - Feb 10 2014

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High Fat Diet
high fat diet
Nutrition
Energy balance
Inbred C57BL Mouse
intestinal microorganisms
energy balance
Fats
Carbohydrates
carbohydrates
mice
Proteins
proteins
Caseins
casein
Leptin
Energy Intake
leptin
Oxygen Consumption
Lactobacillaceae

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Protein quality and the protein to carbohydrate ratio within a high fat diet influences energy balance and the gut microbiota in C57BL/6J mice. / Mcallan, Liam; Skuse, Peter; Cotter, Paul D.; O'Connor, Paula; Cryan, John F.; Ross, R. Paul; Fitzgerald, Gerald; Roche, Helen M.; Nilaweera, Kanishka N.

In: PloS one, Vol. 9, No. 2, e88904, 10.02.2014.

Research output: Contribution to journalArticle

Mcallan, L, Skuse, P, Cotter, PD, O'Connor, P, Cryan, JF, Ross, RP, Fitzgerald, G, Roche, HM & Nilaweera, KN 2014, 'Protein quality and the protein to carbohydrate ratio within a high fat diet influences energy balance and the gut microbiota in C57BL/6J mice', PloS one, vol. 9, no. 2, e88904. https://doi.org/10.1371/journal.pone.0088904
Mcallan, Liam ; Skuse, Peter ; Cotter, Paul D. ; O'Connor, Paula ; Cryan, John F. ; Ross, R. Paul ; Fitzgerald, Gerald ; Roche, Helen M. ; Nilaweera, Kanishka N. / Protein quality and the protein to carbohydrate ratio within a high fat diet influences energy balance and the gut microbiota in C57BL/6J mice. In: PloS one. 2014 ; Vol. 9, No. 2.
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