Proteome of monocytes primed with lipopolysaccharide: Analysis of the abundant proteins

Himanshu S. Gadgil, Karen M. Pabst, Francesco Giorgianni, Edward S. Umstot, Dominic M. Desiderio, Sarka Beranova, Ivan Gerling, Michael J. Pabst

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

We performed a proteomic analysis of monocytes primed by lipopolysaccharide (LPS) in vitro, using two-dimensional gels stained with Coomassie blue. We found 16 proteins of ∼500 detected that either increased or decreased in abundance as a result of priming by LPS (14 with P ≤ 0.05). The proteins were identified by comparing the masses of their tryptic peptides with those of all known proteins, using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and the SWISS-PROT database. Identities were confirmed by matching the sequence of several tryptic peptides, using liquid chromatography electrospray-ionization quadrupole ion trap mass spectrometry. There were increases in the protective enzymes superoxide dismutase and catalase, in four calgranulins, in the cytokine pre-B cell enhancing factor, and in annexin 2, macrophage capping protein, transketolase, pyruvate kinase, and serine/threonine protein kinase 10. Proteins that decreased in abundance were integrin alpha-IIB, protein disulfide isomerase, and platelet-activating factor acetylhydrolase. Many of these altered proteins have interesting functions in inflammation.

Original languageEnglish (US)
Pages (from-to)1767-1780
Number of pages14
JournalProteomics
Volume3
Issue number9
DOIs
StatePublished - Sep 1 2003

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Proteome
Lipopolysaccharides
Monocytes
Proteins
Mass spectrometry
Mass Spectrometry
Leukocyte L1 Antigen Complex
Transketolase
Integrin alpha Chains
Protein Disulfide-Isomerases
Annexins
Electrospray ionization
Protein Databases
Peptides
Pyruvate Kinase
B-Lymphoid Precursor Cells
Macrophages
Platelet Activating Factor
Protein-Serine-Threonine Kinases
Liquid chromatography

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Proteome of monocytes primed with lipopolysaccharide : Analysis of the abundant proteins. / Gadgil, Himanshu S.; Pabst, Karen M.; Giorgianni, Francesco; Umstot, Edward S.; Desiderio, Dominic M.; Beranova, Sarka; Gerling, Ivan; Pabst, Michael J.

In: Proteomics, Vol. 3, No. 9, 01.09.2003, p. 1767-1780.

Research output: Contribution to journalArticle

Gadgil, Himanshu S. ; Pabst, Karen M. ; Giorgianni, Francesco ; Umstot, Edward S. ; Desiderio, Dominic M. ; Beranova, Sarka ; Gerling, Ivan ; Pabst, Michael J. / Proteome of monocytes primed with lipopolysaccharide : Analysis of the abundant proteins. In: Proteomics. 2003 ; Vol. 3, No. 9. pp. 1767-1780.
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