Proteomic analysis of phosphotyrosyl proteins in the rat brain: Effect of butorphanol dependence

Seong Youl Kim, Nuannoi Chudapongse, Sang Lee, Michael C. Levin, Jae Taek Oh, Hae Joon Park, Ing K. Ho

Research output: Contribution to journalArticle

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Abstract

Butorphanol (17-cyclobutylmethyl-3,14-dihydroxymorphinan) tartrate (Stadol) is a mixed agonist-antagonist opioid analgesic agent that is about five to seven times as potent as morphine in analgesic effects. The chronic use of butorphanol produces physical dependence in humans and animals. Phosphorylation plays a very important role in developing butorphanol dependence; however, global phosphorylation events induced by chronic butorphanol administration have not been reported. The aim of this study is to determine the alteration of tyrosine phosphorylation of brain frontal cortical proteins in butorphanol-dependent rats using a proteomic approach. Dependence was produced by continuous intracerebroventricular (i.c.v.) infusion of butorphanol (26 nmol/μl/hr) for 72 hr via osmotic minipump in rats. Similar patterns of protein expression were detected by two-dimensional electrophoresis (2-DE) in brain frontal cortex of butorphanol-dependent and saline-treated control rats. All 65 phosphotyrosyl (p-Tyr) protein spots detected in pH 3-10 phosphotyrosine 2-DE of control rat brains were detected in butorphanol-dependent rat brains. The densities of most p-Tyr protein spots were increased in butorphanol- dependent rat brains compared to saline-treated control samples. Eighteen additional p-Tyr protein spots were detected in pH 3-10 2-DE images of butorphanol-dependent rat brains. Immobilized pH strips with three different narrow pH ranges were examined to improve the resolution of p-Tyr proteins in 2-DE gels. Fifty-three p-Tyr protein spots were identified as known proteins involved in cell cytoskeleton, cell metabolism, and cell signaling. This proteomic approach can provide useful information for understanding the complex mechanism of butorphanol dependence in vivo.

Original languageEnglish (US)
Pages (from-to)867-877
Number of pages11
JournalJournal of Neuroscience Research
Volume77
Issue number6
DOIs
StatePublished - Sep 15 2004

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Butorphanol
Proteomics
Brain
Proteins
Phosphorylation
Analgesics
Intraventricular Infusions
Phosphotyrosine
Frontal Lobe
Cytoskeleton
Morphine
Opioid Analgesics
Tyrosine
Electrophoresis

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience

Cite this

Proteomic analysis of phosphotyrosyl proteins in the rat brain : Effect of butorphanol dependence. / Kim, Seong Youl; Chudapongse, Nuannoi; Lee, Sang; Levin, Michael C.; Oh, Jae Taek; Park, Hae Joon; Ho, Ing K.

In: Journal of Neuroscience Research, Vol. 77, No. 6, 15.09.2004, p. 867-877.

Research output: Contribution to journalArticle

Kim, Seong Youl ; Chudapongse, Nuannoi ; Lee, Sang ; Levin, Michael C. ; Oh, Jae Taek ; Park, Hae Joon ; Ho, Ing K. / Proteomic analysis of phosphotyrosyl proteins in the rat brain : Effect of butorphanol dependence. In: Journal of Neuroscience Research. 2004 ; Vol. 77, No. 6. pp. 867-877.
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