Proteomic Profiling of Exosomes Derived from Plasma of HIV-Infected Alcohol Drinkers and Cigarette Smokers

Sunitha Kodidela, Yujie Wang, Benjamin J. Patters, Yuqing Gong, Namita Sinha, Sabina Ranjit, Kelli Gerth, Sanjana Haque, Theodore Cory, Carole McArthur, Anil Kumar, Jim Y. Wan, Santosh Kumar

Research output: Contribution to journalArticle

Abstract

Abuse of alcohol and tobacco could exacerbate HIV pathogenesis by transferring materials through exosomes (small nanovesicles). Exosomes present a stable and accessible source of information concerning the health and/or disease status of patients, which can provide diagnostic and prognostic biomarkers for myriad conditions. Therefore, we aimed to study the specific exosomal proteins that are altered in both HIV-infected subjects and alcohol/tobacco users. Exosomes were isolated from plasma of the following subjects: a) HIV-negative subjects (healthy), b) HIV-positive subjects (HIV), c) HIV-negative alcohol drinkers (drinkers), d) HIV-negative tobacco smokers (smokers), e) HIV-positive drinkers (HIV + drinkers), and f) HIV-positive smokers (HIV + smokers). Quantitative proteomic profiling was then performed from these exosomes. Sixteen proteins were significantly altered in the HIV group, ten in drinkers, four in HIV + drinkers, and fifteen in smokers compared to healthy subjects. Only one protein, fibulin-1 (FBLN1), was significantly altered in HIV + smokers. Interestingly, hemopexin was not significantly altered in drinkers or HIV patients but was significantly altered in HIV + drinkers. Further, our study is the first to show properdin expression in plasma exosomes, which was decreased in HIV + smokers and HIV + drinkers compared to HIV patients. The present findings suggest that hemopexin and properdin show potential as markers for physiological effects that may arise in HIV-infected individuals who abuse alcohol and tobacco. [Figure not available: see fulltext.].

Original languageEnglish (US)
JournalJournal of Neuroimmune Pharmacology
DOIs
StatePublished - Jan 1 2019

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Exosomes
Tobacco Products
Proteomics
Alcohols
HIV
Tobacco
Hemopexin
Properdin
Alcoholism
Healthy Volunteers

All Science Journal Classification (ASJC) codes

  • Neuroscience (miscellaneous)
  • Immunology and Allergy
  • Immunology
  • Pharmacology

Cite this

Proteomic Profiling of Exosomes Derived from Plasma of HIV-Infected Alcohol Drinkers and Cigarette Smokers. / Kodidela, Sunitha; Wang, Yujie; Patters, Benjamin J.; Gong, Yuqing; Sinha, Namita; Ranjit, Sabina; Gerth, Kelli; Haque, Sanjana; Cory, Theodore; McArthur, Carole; Kumar, Anil; Wan, Jim Y.; Kumar, Santosh.

In: Journal of Neuroimmune Pharmacology, 01.01.2019.

Research output: Contribution to journalArticle

Kodidela, Sunitha ; Wang, Yujie ; Patters, Benjamin J. ; Gong, Yuqing ; Sinha, Namita ; Ranjit, Sabina ; Gerth, Kelli ; Haque, Sanjana ; Cory, Theodore ; McArthur, Carole ; Kumar, Anil ; Wan, Jim Y. ; Kumar, Santosh. / Proteomic Profiling of Exosomes Derived from Plasma of HIV-Infected Alcohol Drinkers and Cigarette Smokers. In: Journal of Neuroimmune Pharmacology. 2019.
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