Pulmonary expression of early growth response-1

Biphasic time course and effect of oxygen concentration

Shi Fang Yan, Jiesheng Lu, Linna Xu, Yu Shan Zou, Joern Tongers, Walter Kisiel, Nigel Mackman, David J. Pinsky, David Stern

Research output: Contribution to journalArticle

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Abstract

Hypoxia induces complex adaptive responses. In this report, induction of early growth response-1 (Egr-1) transcripts in lungs of mice subjected to hypoxia is shown to be dose and time dependent. Within 30 min of hypoxia, Egr-1 transcripts were ~20-fold elevated in 6% oxygen, ~5.2-fold increased by 10% oxygen, and returned to the normoxic baseline by 12% oxygen. Time course studies up to 48 h showed a biphasic profile with an initial steep rise in Egr-1 transcripts after 0.5 h of hypoxia and a second elevation beginning after 20-24 h. Hypoxic induction of Egr-1 was paralleled by enhanced expression of the downstream target gene tissue factor. Egr-1 and tissue factor antigen were visualized in bronchial and vascular smooth muscle and in alveolar macrophages. Egr-1 has the capacity to modulate expression of genes involved in the remodeling of the extracellular matrix and properties of smooth muscle, thus possibly contributing to the pulmonary response to chronic hypoxia.

Original languageEnglish (US)
Pages (from-to)2303-2309+2302
JournalJournal of Applied Physiology
Volume88
Issue number6
StatePublished - Jul 25 2000
Externally publishedYes

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Oxygen
Lung
Growth
Thromboplastin
Alveolar Macrophages
Vascular Smooth Muscle
Extracellular Matrix
Smooth Muscle
Hypoxia
Gene Expression
Antigens
Genes

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Cite this

Yan, S. F., Lu, J., Xu, L., Zou, Y. S., Tongers, J., Kisiel, W., ... Stern, D. (2000). Pulmonary expression of early growth response-1: Biphasic time course and effect of oxygen concentration. Journal of Applied Physiology, 88(6), 2303-2309+2302.

Pulmonary expression of early growth response-1 : Biphasic time course and effect of oxygen concentration. / Yan, Shi Fang; Lu, Jiesheng; Xu, Linna; Zou, Yu Shan; Tongers, Joern; Kisiel, Walter; Mackman, Nigel; Pinsky, David J.; Stern, David.

In: Journal of Applied Physiology, Vol. 88, No. 6, 25.07.2000, p. 2303-2309+2302.

Research output: Contribution to journalArticle

Yan, SF, Lu, J, Xu, L, Zou, YS, Tongers, J, Kisiel, W, Mackman, N, Pinsky, DJ & Stern, D 2000, 'Pulmonary expression of early growth response-1: Biphasic time course and effect of oxygen concentration', Journal of Applied Physiology, vol. 88, no. 6, pp. 2303-2309+2302.
Yan SF, Lu J, Xu L, Zou YS, Tongers J, Kisiel W et al. Pulmonary expression of early growth response-1: Biphasic time course and effect of oxygen concentration. Journal of Applied Physiology. 2000 Jul 25;88(6):2303-2309+2302.
Yan, Shi Fang ; Lu, Jiesheng ; Xu, Linna ; Zou, Yu Shan ; Tongers, Joern ; Kisiel, Walter ; Mackman, Nigel ; Pinsky, David J. ; Stern, David. / Pulmonary expression of early growth response-1 : Biphasic time course and effect of oxygen concentration. In: Journal of Applied Physiology. 2000 ; Vol. 88, No. 6. pp. 2303-2309+2302.
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