Pulmonary macrophage activation in a murine model of the acquired immunodeficiency syndrome (AIDS) is mediated by an interferon ( I F N ) -γ-dependent pathway

C. L. Hartsfield, M. N. Gillespie, Elizabeth Fitzpatrick, M. Avdiushko, P. A. Cohen

Research output: Contribution to journalArticle

Abstract

Despite the hallmark immunosuppression associated with AIDS, there is increasing evidence that lymphocytes are activated in situ. A murine model of AIDS, with immunosuppression evoked by the LP-BM5 retrovi rus, exhibits a diffuse interstitial pneumonitis similar to that seen i n human patients. We used the murine model to assess the activation state of pulmonary lymphocytes and to test the hypothesis that IFN-f contributes to activation of pulmonary macrophages. In LP-BM5-infected mice, flow cytometry demonstrated upregulation of cell surface markers denoting activation of pulmonary lymphocytes and macrophages. Additionally, increased expression of mRNA transcripts for IL-1, IL1θ, TGF-β, and IFN-γ was detected by RT-PCR analysis. Chronic administration of anti-IFN-γ antibodies to immunosuppressed mi ce aboli shed pulmonary macrophage activation. These observations demonstrate that activated lymphoid cells are present in the lungs of mice with LPBM5-induced immunosuppression and suggest a role for IFN-γ -dependent activation of macrophages and subsequent release of macrophage-derived cytokines in the pathogenesis of noninfectious pulmonary disease in AIDS. (Supported by NIH).

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number3
StatePublished - 1996
Externally publishedYes

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Murine Acquired Immunodeficiency Syndrome
macrophage activation
acquired immunodeficiency syndrome
Macrophage Activation
Alveolar Macrophages
interferons
Immunosuppression
Interferons
animal models
Chemical activation
lungs
Lymphocytes
Macrophages
immunosuppression
Acquired Immunodeficiency Syndrome
macrophages
lymphocytes
Lung
Interstitial Lung Diseases
Lymphocyte Activation

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Pulmonary macrophage activation in a murine model of the acquired immunodeficiency syndrome (AIDS) is mediated by an interferon ( I F N ) -γ-dependent pathway. / Hartsfield, C. L.; Gillespie, M. N.; Fitzpatrick, Elizabeth; Avdiushko, M.; Cohen, P. A.

In: FASEB Journal, Vol. 10, No. 3, 1996.

Research output: Contribution to journalArticle

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