Pulmonary metabolism of prostacyclin (PGI2) in the rabbit

P. Y K Wong, J. C. McGiff, F. F. Sun, Kafait Malik

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Metabolism of prostacyclin, [9-3H]PGI2, was examined in the isolated perfused rabbit lung and the post-microsomal supernate of rabbit lung homogenate. Two major metabolites of [9-3H]PGI2 from the lung perfusate were separated by thin-layer chromatography and radiometric gas-chromatography. These two products were identified as 6 keto-PGF and 6,15 diketo-13,14 dihydro PGF by mass-spectrometry; they represented 65% and 14% of the total radioactivity. When [9-3H]PGI2 was incubated with the lung homogenate in the presence of either NAD+ or NADP+, more than 36% and 25%, respectively, was converted to the 6,15 diketo-13,14 dihydro metabolite.

Original languageEnglish (US)
Pages (from-to)731-738
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume83
Issue number2
DOIs
StatePublished - Jul 28 1978
Externally publishedYes

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Epoprostenol
Metabolism
Rabbits
Lung
Metabolites
Thin layer chromatography
Radioactivity
Thin Layer Chromatography
NADP
Gas chromatography
Gas Chromatography
NAD
Mass spectrometry
Mass Spectrometry
prostaglandin F1

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Pulmonary metabolism of prostacyclin (PGI2) in the rabbit. / Wong, P. Y K; McGiff, J. C.; Sun, F. F.; Malik, Kafait.

In: Biochemical and Biophysical Research Communications, Vol. 83, No. 2, 28.07.1978, p. 731-738.

Research output: Contribution to journalArticle

Wong, P. Y K ; McGiff, J. C. ; Sun, F. F. ; Malik, Kafait. / Pulmonary metabolism of prostacyclin (PGI2) in the rabbit. In: Biochemical and Biophysical Research Communications. 1978 ; Vol. 83, No. 2. pp. 731-738.
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