Purification of DNA complementary to nucleotide sequences required for neoplastic transformation of fibroblasts by avian sarcoma viruses

Dominique Stehelin, Ramareddy Guntaka, Harold E. Varmus, J. Michael Bishop

Research output: Contribution to journalArticle

131 Citations (Scopus)

Abstract

We have prepared radioactive DNA (cDNA sarc ) complementary to nucleotide sequences which represent at least a portion of the viral gene(s) required for neoplastic transformation of fibroblasts by an avian sarcoma virus. The genetic complexity of cDNA sarc (~1600 nucleotides) is sufficient to represent an entire cistron. The genomes of three independent isolates of avian sarcoma viruses share nucleotide sequences closely related to cDNA sarc , whereas the sequences are absent from transformation-defective mutants of avian sarcoma viruses, several avian leukosis viruses, a non-pathogenic endogenous virus of chickens (Rous-associated virus-O), sarcoma-leukosis viruses of mice and cats, and mouse mammary tumor virus. We conclude that the transforming gene(s) of all avian sarcoma viruses have closely related or common genetic lineages distinct from the transforming genes in sarcoma viruses of other species. Our results conform to previous reports that transformation-defective variants of avian sarcoma viruses are mutants with identical regions deleted from each subunit of a polyploid genome.

Original languageEnglish (US)
Pages (from-to)349-365
Number of pages17
JournalJournal of Molecular Biology
Volume101
Issue number3
DOIs
StatePublished - Mar 5 1976
Externally publishedYes

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Avian Sarcoma Viruses
Complementary DNA
Fibroblasts
Avian Leukosis Virus
Oncogenes
Murine Sarcoma Viruses
Genome
Viruses
Mouse mammary tumor virus
Polyploidy
Viral Genes
Sarcoma
Chickens
Cats
Nucleotides
DNA
Genes

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

Cite this

Purification of DNA complementary to nucleotide sequences required for neoplastic transformation of fibroblasts by avian sarcoma viruses. / Stehelin, Dominique; Guntaka, Ramareddy; Varmus, Harold E.; Bishop, J. Michael.

In: Journal of Molecular Biology, Vol. 101, No. 3, 05.03.1976, p. 349-365.

Research output: Contribution to journalArticle

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