Quantifying single microvessel permeability in isolated blood-perfused rat lung preparation

Kathirvel Kandasamy, Kaushik Parthasarathi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The isolated blood-perfused lung preparation is widely used to visualize and define signaling in single microvessels. By coupling this preparation with real time imaging, it becomes feasible to determine permeability changes in individual pulmonary microvessels. Herein we describe steps to isolate rat lungs and perfuse them with autologous blood. Then, we outline steps to infuse fluorophores or agents via a microcatheter into a small lung region. Using these procedures described, we determined permeability increases in rat lung microvessels in response to infusions of bacterial lipopolysaccharide. The data revealed that lipopolysaccharide increased fluid leak across both venular and capillary microvessel segments. Thus, this method makes it possible to compare permeability responses among vascular segments and thus, define any heterogeneity in the response. While commonly used methods to define lung permeability require postprocessing of lung tissue samples, the use of real time imaging obviates this requirement as evident from the present method. Thus, the isolated lung preparation combined with real time imaging offers several advantages over traditional methods to determine lung microvascular permeability, yet is a straightforward method to develop and implement.

Original languageEnglish (US)
Pages (from-to)e51552
JournalJournal of Visualized Experiments
Issue number88
DOIs
StatePublished - 2014

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Microvessels
Rats
Permeability
Blood
Imaging techniques
Lung
Lipopolysaccharides
Fluorophores
Tissue
Fluids
Capillary Permeability
Blood Vessels

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Quantifying single microvessel permeability in isolated blood-perfused rat lung preparation. / Kandasamy, Kathirvel; Parthasarathi, Kaushik.

In: Journal of Visualized Experiments, No. 88, 2014, p. e51552.

Research output: Contribution to journalArticle

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