Quantitation of cocaine and cocaethylene in canine serum by high-performance liquid chromatography

Cheri L. Williams, Steven Laizure, Robert Parker, John J. Lima

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A reversed-phase high-performance liquid chromatographic procedure for the determination of cocaine and cocaethylene in canine serum has been developed. The compounds were extracted from 1 ml of alkalinized canine serum with hexane. Chromatographic separation was achieved with a cyanopropyl column (250 x 4.6 mm I.D., 5 μm) using a mobile phase of acetonitrile and phosphate buffer, pH 7.40 (38:62, v/v) flowing at 1 ml/min. Eluate was monitored by a variable-wavelength UV detector set to 230 nm. The extraction procedure yields an average recovery of 99 and 96% for cocaine and cocaethylene, respectively. The between-day coefficients of variation, at 2400 ng/ml, for cocaine and cocaethylene were both 8.6% and the within-day coefficients of variation, at 400 ng/ml, for cocaine and cocaethylene were 7.3 and 8.0%, respectively. A concentration-time profile resulting from administration of 3 mg/kg cocaine and cocaethylene to the dog revealed a similar disposition between cocaine and cocaethylene, with a clearance and volume of distribution at steady-state values of 72.8 and 61.0 ml/min/kg and 2.6 and 2.7 l/kg, respectively.

Original languageEnglish (US)
Pages (from-to)271-276
Number of pages6
JournalJournal of Chromatography B: Biomedical Applications
Volume681
Issue number2
DOIs
StatePublished - Jun 7 1996

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High performance liquid chromatography
Cocaine
Ultraviolet detectors
Hexanes
cocaethylene
Buffers
Phosphates
Recovery
Wavelength
Liquids

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

Quantitation of cocaine and cocaethylene in canine serum by high-performance liquid chromatography. / Williams, Cheri L.; Laizure, Steven; Parker, Robert; Lima, John J.

In: Journal of Chromatography B: Biomedical Applications, Vol. 681, No. 2, 07.06.1996, p. 271-276.

Research output: Contribution to journalArticle

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