Quantitative analysis of nanoscale intranuclear structural alterations in hippocampal cells in chronic alcoholism via transmission electron microscopy imaging

Peeyush Sahay, Pradeep Kumar Shukla, Hemendra M. Ghimire, Huda M. Almabadi, Vibha Tripathi, Samarendra K. Mohanty, Radhakrishna Rao, Prabhakar Pradhan

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Chronic alcoholism is known to alter the morphology of the hippocampus, an important region of cognitive function in the brain. Therefore, to understand the effect of chronic alcoholism on hippocampal neural cells, we employed a mouse model of chronic alcoholism and quantified intranuclear nanoscale structural alterations in these cells. Transmission electron microscopy (TEM) images of hippocampal neurons were obtained, and the degree of structural alteration in terms of mass density fluctuation was determined using the light-localization properties of optical media generated from TEM imaging. The results, which were obtained at length scales ranging from ∼30 to 200 nm, show that 10-12 week-old mice fed a Lieber-DeCarli liquid (alcoholic) diet had a higher degree of structural alteration than control mice fed a normal diet without alcohol. The degree of structural alteration became significantly distinguishable at a sample length of ∼100 nm, which is the typical length scale of the building blocks of cells, such as DNA, RNA, proteins and lipids. Interestingly, different degrees of structural alteration at such length scales suggest possible structural rearrangement of chromatin inside the nuclei in chronic alcoholism.

Original languageEnglish (US)
Article number026001
JournalPhysical Biology
Volume14
Issue number2
DOIs
StatePublished - Mar 1 2017

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Transmission Electron Microscopy
Alcoholism
Diet
Cognition
Chromatin
Hippocampus
Alcohols
RNA
Lipids
Neurons
Light
DNA
Brain
Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Molecular Biology
  • Cell Biology

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Quantitative analysis of nanoscale intranuclear structural alterations in hippocampal cells in chronic alcoholism via transmission electron microscopy imaging. / Sahay, Peeyush; Shukla, Pradeep Kumar; Ghimire, Hemendra M.; Almabadi, Huda M.; Tripathi, Vibha; Mohanty, Samarendra K.; Rao, Radhakrishna; Pradhan, Prabhakar.

In: Physical Biology, Vol. 14, No. 2, 026001, 01.03.2017.

Research output: Contribution to journalArticle

Sahay, Peeyush ; Shukla, Pradeep Kumar ; Ghimire, Hemendra M. ; Almabadi, Huda M. ; Tripathi, Vibha ; Mohanty, Samarendra K. ; Rao, Radhakrishna ; Pradhan, Prabhakar. / Quantitative analysis of nanoscale intranuclear structural alterations in hippocampal cells in chronic alcoholism via transmission electron microscopy imaging. In: Physical Biology. 2017 ; Vol. 14, No. 2.
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