Radioimmunoassay detection of endorphins from long-term culture of human pituitary tumour cells

Burt Sharp, S. Melmed, R. Goldberg, H. E. Carlson, S. Refetoff, J. M. Hershman

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Using a sensitive and precise radioimmunoassay for human β-endorphin, we have demonstrated the sustained secretion of opioid peptides from human pituitary tumour cells. Pituitary tumour tissue obtained from a patient with Nelson's syndrome was maintained in continuous monolayer culture and secreted both β-lipotropin and β-endorphin, with predominance of the latter. This is compatible with the idea that the β-endorphin in normal human serum is secreted as such despite the predominance of β-lipoprotein compared with β-endorphin in the anterior pituitary.

Original languageEnglish (US)
Pages (from-to)174-178
Number of pages5
JournalActa Endocrinologica
Volume99
Issue number2
StatePublished - Mar 15 1982

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Endorphins
Pituitary Neoplasms
Radioimmunoassay
Nelson Syndrome
beta-Lipotropin
Opioid Peptides
Lipoproteins
Serum

All Science Journal Classification (ASJC) codes

  • Endocrinology

Cite this

Sharp, B., Melmed, S., Goldberg, R., Carlson, H. E., Refetoff, S., & Hershman, J. M. (1982). Radioimmunoassay detection of endorphins from long-term culture of human pituitary tumour cells. Acta Endocrinologica, 99(2), 174-178.

Radioimmunoassay detection of endorphins from long-term culture of human pituitary tumour cells. / Sharp, Burt; Melmed, S.; Goldberg, R.; Carlson, H. E.; Refetoff, S.; Hershman, J. M.

In: Acta Endocrinologica, Vol. 99, No. 2, 15.03.1982, p. 174-178.

Research output: Contribution to journalArticle

Sharp, B, Melmed, S, Goldberg, R, Carlson, HE, Refetoff, S & Hershman, JM 1982, 'Radioimmunoassay detection of endorphins from long-term culture of human pituitary tumour cells', Acta Endocrinologica, vol. 99, no. 2, pp. 174-178.
Sharp B, Melmed S, Goldberg R, Carlson HE, Refetoff S, Hershman JM. Radioimmunoassay detection of endorphins from long-term culture of human pituitary tumour cells. Acta Endocrinologica. 1982 Mar 15;99(2):174-178.
Sharp, Burt ; Melmed, S. ; Goldberg, R. ; Carlson, H. E. ; Refetoff, S. ; Hershman, J. M. / Radioimmunoassay detection of endorphins from long-term culture of human pituitary tumour cells. In: Acta Endocrinologica. 1982 ; Vol. 99, No. 2. pp. 174-178.
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