Radioimmunoassay of fibrinogen-fibrin degradation products

Assay for fragment E-related neoantigen -methodological aspects

James Chen, Richard S. Schulof

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

E fragment and its antigenically-related degradation products of human fibrinogen (Fg) and/or fibrin (Fb) have been measured in both normal and pathological plasmas by a specific radioimmunoassay (RIA) for the neoantigenic expression of E(Eneo) which is engendered after the plasmic cleavage of Fg or Fb. High titer antisera were produced in rabbits against Fg-E (E derived from Fg). The anti-Fg-E sera harvested were absorbed with a cyanogen bromide-activated immunoadsorbent column coupled with intact Fg. The Fg-absorbed antisera were employed to develop a double antibody RIA which detected < 1 ng/ml of Fg-E and exhibited little cross-reactivity of Fg at 1 mg/ml. This Eneo RIA did not discriminate between Fg-E and Fb-E (E derived from Fb) in the competitive inhibition profile, and hence it measured total Eneo antigens. The displacement curves of normal plasmas generally showed a parallel relationship to the standard which permitted the calculation of normal plasma Eneo immunoreactivity (ca. 15 ng/ml). Considerably higher plasma Eneo immunoreactivities have been observed in patients with consumption coagulopathy. Modifications of the Eneo RIA which permit the attainment of this sensitivity are described.

Original languageEnglish (US)
Pages (from-to)601-615
Number of pages15
JournalThrombosis Research
Volume16
Issue number5-6
DOIs
StatePublished - Jan 1 1979

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Fibrin Fibrinogen Degradation Products
Fibrinogen
Radioimmunoassay
Fibrin
Immune Sera
Cyanogen Bromide
Immunosorbents
Disseminated Intravascular Coagulation
Rabbits

All Science Journal Classification (ASJC) codes

  • Hematology

Cite this

Radioimmunoassay of fibrinogen-fibrin degradation products : Assay for fragment E-related neoantigen -methodological aspects. / Chen, James; Schulof, Richard S.

In: Thrombosis Research, Vol. 16, No. 5-6, 01.01.1979, p. 601-615.

Research output: Contribution to journalArticle

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